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Lab-on-DVD :
Lab-on-DVD : standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics
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- Ramachandraiah, Harisha (författare)
- KTH,Proteomik,Science for Life Laboratory, SciLifeLab
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- Amasia, Mary (författare)
- KTH,Science for Life Laboratory, SciLifeLab
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Cole, Jackie (författare)
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Sheard, Paul (författare)
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Pickhaver, Simon (författare)
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Walker, Chris (författare)
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Wirta, Valtteri (författare)
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Lexow, Preben (författare)
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Lione, Richard (författare)
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- Russom, Aman (författare)
- KTH,Proteomik,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- Royal Society of Chemistry (RSC), 2013
- 2013
- Engelska.
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Ingår i: Lab on a Chip. - : Royal Society of Chemistry (RSC). - 1473-0197 .- 1473-0189. ; 13:8, s. 1578-1585
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We present a novel "Lab-on-DVD" system and demonstrate its capability for rapid and low-cost HIV diagnostics by counting CD4+ cells isolated from whole blood. We show that a commercial DVD drive can, with certain modifications, be turned into an improved DVD-based laser scanning microscope (DVD-LSM). The system consists of a multi-layered disposable polymer disc and a modified commercial DVD reader with rotational control for sample handling, temperature control for optimized bioassay, a photodiode array for detection, and software for signal processing and user interface - all the necessary components required for a truly integrated lab-on-a-chip system, with the capability to deliver high-resolution images down to 1 mm in size. Using discs modified with antibodies, we specifically captured CD4+ cells from whole blood, demonstrating single cell resolution imaging. The novel integrated DVD platform with sub-micron image resolution brings, for the first time, affordable cellular diagnostic testing to the point-of-care and should be readily applicable at resource-limited settings.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Platform
- Microchip
- Device
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Ramachandraiah, ...
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Amasia, Mary
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Cole, Jackie
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Sheard, Paul
-
Pickhaver, Simon
-
Walker, Chris
-
visa fler...
-
Wirta, Valtteri
-
Lexow, Preben
-
Lione, Richard
-
Russom, Aman
-
visa färre...
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- NATURVETENSKAP
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Lab on a Chip
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