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Triplet-State Inves...
Triplet-State Investigations of Fluorescent Dyes at Dielectric Interfaces Using Total Internal Reflection Fluorescence Correlation Spectroscopy
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- Blom, Hans (författare)
- KTH,Experimentell biomolekylär fysik
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- Chmyrov, Andriy, 1979- (författare)
- KTH,Experimentell biomolekylär fysik
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- Hassler, Kai (författare)
- KTH,Experimentell biomolekylär fysik
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Davis, L.M. (författare)
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- Widengren, Jerker (författare)
- KTH,Experimentell biomolekylär fysik
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(creator_code:org_t)
- 2009-04-17
- 2009
- Engelska.
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Ingår i: Journal of Physical Chemistry A. - Washington, DC : American Chemical Society. - 1089-5639 .- 1520-5215. ; 113:19, s. 5554-5566
- Relaterad länk:
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http://pubs.acs.org/...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The triplet-state kinetics of several fluorescent dyes used in ultrasensitive fluorescence microscopy are investigated using total internal reflection fluorescence correlation spectroscopy (TIR-FCS). A theoretical outline of the correlation analysis and the physical aspects of evanescent excitation and fluorescence emission at dielectric interfaces are given. From this analysis, the rates of intersystem crossing and triplet decay are deduced for fluorescein, ATTO 488, rhodamine 110, rhodamine 123, and rhodamine 6G in aqueous buffer solutions. All investigated dyes show slightly higher triplet rates at the dielectric interface compared to bulk solution measurements. We attribute this enhancement to possible modifications of the dyes’ photophysical properties near a dielectric interface. In the case of rhodamine 6G, the impact of changes in the dye concentration, ionic strength of the solvent, and potassium iodide concentration are also investigated. This leads to a better understanding of the influences of dye−dye, dye−solvent, and dye−surface interactions on the increased triplet intersystem crossing and triplet decay rates. The study shows that analysis of triplet-state kinetics by TIR-FCS not only results in a better understanding of how the photophysical properties of the dyes are affected by the presence of an interface, but also provides a means for probing the microenvironment near dielectric interfaces.
Ämnesord
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
Nyckelord
- fluorescence correlation spectroscopy
- FCS
- triplet state
- surface effects
- TIR
- TIR-FCS
- total internal reflection
- Spectroscopy
- Spektroskopi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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