Sökning: onr:"swepub:oai:DiVA.org:kth-123643" >
Genotyping of singl...
Genotyping of single nucleotide polymorphisms by melting curve analysis using thin film semi-transparent heaters integrated in a lab-on-foil system
-
Ohlander, Anna (författare)
-
Zilio, Caterina (författare)
-
Hammerle, Tobias (författare)
-
visa fler...
-
- Zelenin, Sergey (författare)
- KTH,Proteomik,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
-
Klink, Gerhard (författare)
-
Chiari, Marcella (författare)
-
Bock, Karlheinz (författare)
-
- Russom, Aman (författare)
- KTH,Proteomik,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
-
visa färre...
-
(creator_code:org_t)
- Royal Society of Chemistry (RSC), 2013
- 2013
- Engelska.
-
Ingår i: Lab on a Chip. - : Royal Society of Chemistry (RSC). - 1473-0197 .- 1473-0189. ; 13:11, s. 2075-2082
- Relaterad länk:
-
https://kth.diva-por... (primary) (Raw object)
-
visa fler...
-
https://pubs.rsc.org...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The recent technological advances in micro/nanotechnology present new opportunities to combine microfluidics with microarray technology for the development of small, sensitive, single-use, point-of-care molecular diagnostic devices. As such, the integration of microarray and plastic microfluidic systems is an attractive low-cost alternative to glass based microarray systems. This paper presents the integration of a DNA microarray and an all-polymer microfluidic foil system with integrated thin film heaters, which demonstrate DNA analysis based on melting curve analysis (MCA). A novel micro-heater concept using semi-transparent copper heaters manufactured by roll-to-roll and lift-off on polyethylene naphthalate (PEN) foil has been developed. Using a mesh structure, heater surfaces have been realized in only one single metallization step, providing more efficient and homogenous heating characteristics than conventional meander heaters. A robust DNA microarray spotting protocol was adapted on Parylene C coated heater-foils, using co-polymer poly(DMA-NAS-MAPS) to enable covalent immobilization of DNA. The heaters were integrated in a microfluidic channel using lamination foils and MCA of the spotted DNA duplexes showed single based discrimination of mismatched over matched target DNA-probes. Finally, as a proof of principle, we perform MCA on PCR products to detect the Leu7Pro polymorphism of the neutropeptide Y related to increased risk of Type II diabetes, BMI and depression.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- article
- body mass
- depression
- devices
- DNA determination
- DNA microarray
- film
- foil
- heating
- melting curve analysis
- non insulin dependent diabetes mellitus
- nucleic acid analysis
- polymerase chain reaction
- priority journal
- single nucleotide polymorphism
- thin film heater
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas