Affinity ligands for industrial protein purification

• Significant efforts are put into the design of large-scale purification processes of proteins due to great demands regarding cost efficiency and safety. In order to design an effective purification scheme the unit operations need to be reduced to a minimum. In this review we are discussing proteinaceous ligands as well as small synthetic mimics for use in affinity chromatography for large-scale applications. Different advantages as well as drawbacks of the two approaches are outlined.

Keyword

Affinity chromatography

cleaning-in-place (CIP)

deamidation

protein A

protein G

protein engineering

stabilization

escherichia-coli

synthetic ligand

deamidation

chromatography

peptides

residues

stability

design

immobilization

mutagenesis

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