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Scanning inverse fl...
Scanning inverse fluorescence correlation spectroscopy
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- Bergstrand, Jan (författare)
- KTH,Experimentell biomolekylär fysik
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- Rönnlund, Daniel (författare)
- KTH,Experimentell biomolekylär fysik
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- Widengren, Jerker, 1965- (författare)
- KTH,Experimentell biomolekylär fysik
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- Wennmalm, Stefan (författare)
- KTH,Experimentell biomolekylär fysik,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- Optical Society of America, 2014
- 2014
- Engelska.
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Ingår i: Optics Express. - : Optical Society of America. - 1094-4087. ; 22:11, s. 13073-13090
- Relaterad länk:
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https://kth.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Scanning Inverse Fluorescence Correlation Spectroscopy (siFCS) is introduced to determine the absolute size of nanodomains on surfaces. We describe here equations for obtaining the domain size from cross-and auto-correlation functions, measurement simulations which enabled testing of these equations, and measurements on model surfaces mimicking membranes containing nanodomains. Using a confocal microscope of 270 nm resolution the size of 250 nm domains were estimated by siFCS to 257 +/- 12 nm diameter, and 40 nm domains were estimated to 65 +/- 26 nm diameter. Applications of siFCS for sizing of nanodomains and protein clusters in cell membranes are discussed.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Nyckelord
- Image Correlation Spectroscopy
- Cross-Correlation Spectroscopy
- Stimulated-Emission
- Microscopy
- Nanoscopy
- Proteins
- Cells
- Limit
- Biological Physics
- Biologisk fysik
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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