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Slipdisc :
Slipdisc : A versatile sample preparation platform for point of care diagnostics
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- Banerjee, Indradumna (författare)
- KTH,Proteomik och nanobioteknologi
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- Salih, Tagrid (författare)
- KTH,Proteomik och nanobioteknologi
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- Ramachandraiah, Harisha (författare)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Erlandsson, Johan (författare)
- KTH,Fiberteknologi
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- Pettersson, Torbjörn (författare)
- KTH,Wallenberg Wood Science Center,Fiber- och polymerteknologi
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Araújo, A. C. (författare)
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Karlsson, M. (författare)
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- Russom, Aman, Assoc.Prof. (författare)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- 2017
- 2017
- Engelska.
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Ingår i: RSC Advances. - : Royal Society of Chemistry. - 2046-2069. ; 7:56, s. 35048-35054
- Relaterad länk:
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https://doi.org/10.1...
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https://pubs.rsc.org...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We report a microfluidic sample preparation platform called "Slipdisc" based on slipchip technology. Slipdisc is a rotational slipchip that uses a unique hand-wound clockwork mechanism for precise movement of specially fabricated polycarbonate discs. In operation, the microchannels and microchambers carved on the closely aligned microfluidic discs convert from continuous filled paths to defined compartments using the slip movement. The clockwork mechanism introduced here is characterised by a food dye experiment and a conventional HRP TMB reaction before measuring lactate dehydrogenase (LDH) enzyme levels, which is a crucial biomarker for neonatal diagnostics. The colorimetry based detection of LDH was performed with an unmodified camera and an image analysis procedure based on normalising images and observing changes in red channel intensity. The analysis showed a close to unity coefficient of determination (R2 = 0.96) in detecting the LDH concentration when compared with a standard Chemical Analyser, demonstrating the excellent performance of the slipdisc platform with colorimetric detection. The versatile point of care sample preparation platform should ideally be suited for a multitude of applications at resource-limited settings.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- Chemical analysis
- Chemical detection
- Clocks
- Colorimetric analysis
- Colorimetry
- Coefficient of determination
- Colorimetric detection
- Lactate dehydrogenase
- Micro-chambers
- Point of care
- Point of care diagnostic
- Red channels
- Sample preparation
- Microfluidics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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