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Multiple-primer DNA...
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Gharizadeh, B.
(författare)
Multiple-primer DNA sequencing method
- Artikel/kapitelEngelska2003
Förlag, utgivningsår, omfång ...
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Wiley,2003
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:kth-22479
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-22479URI
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https://doi.org/10.1002/elps.200390147DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1928807URI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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QC 20100525
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A multiple-primer DNA sequencing approach suitable for genotyping, detection and identification of microorganisms and viruses has been developed. In this new method two or m ore sequencing primers, combined in a pool, are added to a DNA sample of interest. The oligonucleotide that hybridizes to the DNA sample will function as a primer during the subsequent DNA sequencing procedure. This strategy is suited for selective detection and genotyping of relevant microorganisms and samples harboring different DNA targets such as,multiple variant/infected samples as well as unspecific amplification products. This method is used here in a model system for detection and typing of high-risk oncogenic human papilloma viruses (HPVs) in samples containing multiple infections/variants or unspecific amplification products. Type-specific sequencing primers were designed for four of the most oncogenic (high-risk) HPV types (HPV-16, HPV-18, HPV-33, and HPV-45). The primers were combined and added to a sample containing a mixture of one high-risk (16, 18, 33, or 45) and one or two low-risk types. The DNA samples were sequenced by the Pyrosequencing(TM) technology and the Sanger dideoxy sequencing method. Correct genotyping was achieved in all tested combinations. This multiple-sequencing primer approach also improved the sequence data quality for samples containing unspecific amplification products. The new strategy is highly suitable for diagnostic typing of relevant species/genotypes of microorganisms.
Ämnesord och genrebeteckningar
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human papilloma virus
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microorganisms
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multiple infections
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multiple sequencing primers
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pyrosequencing
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ribosomal database project
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human-papillomavirus dna
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cervical-cancer
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prevalence
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women
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association
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carcinoma
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infection
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lesions
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Ghaderi, M.Karolinska Institutet
(författare)
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Donnelly, D.
(författare)
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Amini, B.
(författare)
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Wallin, K. L.Karolinska Institutet
(författare)
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Nyrén, PålKTH,Biokemi och biokemisk teknologi(Swepub:kth)u134qr82
(författare)
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Karolinska InstitutetBiokemi och biokemisk teknologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Electrophoresis: Wiley24:08-jul, s. 1145-11510173-08351522-2683
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