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Super-resolution mi...
Super-resolution microscopy can identify specific protein distribution patterns in platelets incubated with cancer cells
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- Bergstrand, Jan (author)
- KTH,Kvant- och biofotonik,Royal Inst Technol KTH, Dept Appl Phys, Albanova Univ Ctr, Expt Biomol Phys, SE-10691 Stockholm, Sweden
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- Xu, Lei (author)
- KTH,Kvant- och biofotonik,Royal Inst Technol KTH, Dept Appl Phys, Albanova Univ Ctr, Expt Biomol Phys, SE-10691 Stockholm, Sweden.
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- Miao, Xinyan (author)
- KTH,Kvant- och biofotonik,Royal Inst Technol KTH, Dept Appl Phys, Albanova Univ Ctr, Expt Biomol Phys, SE-10691 Stockholm, Sweden.
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- Li, Nailin (author)
- Karolinska Institutet
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- Öktem, Ozan, 1969- (author)
- KTH,Matematik (Avd.),Royal Inst Technol KTH, Dept Math, Lindstedsvagen 25, SE-10044 Stockholm, Sweden
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- Franzen, Bo (author)
- Karolinska Institutet
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- Auer, Gert (author)
- Karolinska Institutet
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- Lomnytska, Marta (author)
- Karolinska Inst, Dept Oncol Pathol, Karolinska Univ Hosp, K7,Z1 00, S-17176 Stockholm, Sweden.;Acad Univ Hosp, Dept Obstet & Gynaecol, SE-75185 Uppsala, Sweden.;Uppsala Univ, Inst Women & Child Hlth, SE-75185 Uppsala, Sweden.
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- Widengren, Jerker (author)
- KTH,Kvant- och biofotonik,Royal Inst Technol KTH, Dept Appl Phys, Albanova Univ Ctr, Expt Biomol Phys, SE-10691 Stockholm, Sweden
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(creator_code:org_t)
- 2019
- 2019
- English.
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In: Nanoscale. - : ROYAL SOC CHEMISTRY. - 2040-3364 .- 2040-3372. ; 11:20, s. 10023-10033
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Abstract
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- Protein contents in platelets are frequently changed upon tumor development and metastasis. However, how cancer cells can influence protein-selective redistribution and release within platelets, thereby promoting tumor development, remains largely elusive. With fluorescence-based super-resolution stimulated emission depletion (STED) imaging we reveal how specific proteins, implicated in tumor progression and metastasis, re-distribute within platelets, when subject to soluble activators (thrombin, adenosine diphosphate and thromboxane A2), and when incubated with cancer (MCF-7, MDA-MB-231, EFO21) or non-cancer cells (184A1, MCF10A). Upon cancer cell incubation, the cell-adhesion protein P-selectin was found to re-distribute into circular nano-structures, consistent with accumulation into the membrane of protein-storing alpha-granules within the platelets. These changes were to a significantly lesser extent, if at all, found in platelets incubated with normal cells, or in platelets subject to soluble platelet activators. From these patterns, we developed a classification procedure, whereby platelets exposed to cancer cells, to non-cancer cells, soluble activators, as well as non-activated platelets all could be identified in an automatic, objective manner. We demonstrate that STED imaging, in contrast to electron and confocal microscopy, has the necessary spatial resolution and labelling efficiency to identify protein distribution patterns in platelets and can resolve how they specifically change upon different activations. Combined with image analyses of specific protein distribution patterns within the platelets, STED imaging can thus have a role in future platelet-based cancer diagnostics and therapeutic monitoring. The presented approach can also bring further clarity into fundamental mechanisms for cancer cell-platelet interactions, and into non-contact cell-to-cell interactions in general.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
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- art (subject category)
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Nanoscale
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- By the author/editor
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Bergstrand, Jan
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Xu, Lei
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Miao, Xinyan
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Li, Nailin
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Öktem, Ozan, 196 ...
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Franzen, Bo
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show more...
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Auer, Gert
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Lomnytska, Marta
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Widengren, Jerke ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
- Articles in the publication
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Nanoscale
- By the university
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Royal Institute of Technology
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Uppsala University
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Karolinska Institutet