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Electrospray ioniza...
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Ek, PatrikKTH,Analytisk kemi
(author)
Electrospray ionization mass spectrometry from discrete nanoliter-sized sample volumes
- Article/chapterEnglish2010
Publisher, publication year, extent ...
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2010-07-27
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Wiley,2010
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-26071
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-26071URI
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https://doi.org/10.1002/rcm.4674DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20101111
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We describe a method for nanoelectrospray ionization mass spectrometry (nESI-MS) of very small sample volumes. Nanoliter-sized sample droplets were taken up by suction into a nanoelectrospray needle from a silicon microchip prior to ESI. To avoid a rapid evaporation of the small sample volumes, all manipulation steps were performed under a cover of fluorocarbon liquid. Sample volumes down to 1.5 nL were successfully analyzed, and an absolute limit of detection of 105 attomole of insulin (chain B, oxidized) was obtained. The open access to the sample droplets on the silicon chip provides the possibility to add reagents to the sample droplets and perform chemical reactions under an extended period of time. This was demonstrated in an example where we performed a tryptic digestion of cytochrome C in a nanoliter-sized sample volume for 2.5h, followed by monitoring the outcome of the reaction with nESI-MS. The technology was also utilized for tandem mass spectrometry (MS/MS) sequencing analysis of a 2 nL solution of angiotensin I.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Stjernström, MårtenKTH,Analytisk kemi(Swepub:kth)u11asu0n
(author)
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Emmer, ÅsaKTH,Analytisk kemi(Swepub:kth)u1czoysx
(author)
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Roeraade, JohanKTH,Analytisk kemi(Swepub:kth)u10i2qgl
(author)
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KTHAnalytisk kemi
(creator_code:org_t)
Related titles
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In:Rapid Communications in Mass Spectrometry: Wiley24:17, s. 2561-25680951-41981097-0231
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