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Automation of Spati...
Automation of Spatial Transcriptomics library preparation to enable rapid and robust insights into spatial organization of tissues
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- Berglund, Emelie (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Saarenpää, Sami (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Jemt, Anders (författare)
- Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Sci Life Lab, Stockholm, Sweden
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- Gruselius, Joel (författare)
- Karolinska Inst, Dept Biosci & Nutr, Sci Life Lab, Stockholm, Sweden
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- Larsson, Ludvig (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Bergenstråhle, Ludvig (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Lundeberg, Joakim (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Genteknologi
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- Giacomello, Stefania (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Genteknologi
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(creator_code:org_t)
- 2020-04-15
- 2020
- Engelska.
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Ingår i: BMC Genomics. - : Springer Nature. - 1471-2164. ; 21:1
- Relaterad länk:
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https://doi.org/10.1...
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https://kth.diva-por... (primary) (Raw object)
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https://bmcgenomics....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Background: Interest in studying the spatial distribution of gene expression in tissues is rapidly increasing. Spatial Transcriptomics is a novel sequencing-based technology that generates high-throughput information on the distribution, heterogeneity and co-expression of cells in tissues. Unfortunately, manual preparation of high-quality sequencing libraries is time-consuming and subject to technical variability due to human error during manual pipetting, which results in sample swapping and the accidental introduction of batch effects. All these factors complicate the production and interpretation of biological datasets.Results: We have integrated an Agilent Bravo Automated Liquid Handling Platform into the Spatial Transcriptomics workflow. Compared to the previously reported Magnatrix 8000+ automated protocol, this approach increases the number of samples processed per run, reduces sample preparation time by 35%, and minimizes batch effects between samples. The new approach is also shown to be highly accurate and almost completely free from technical variability between prepared samples.Conclusions: The new automated Spatial Transcriptomics protocol using the Agilent Bravo Automated Liquid Handling Platform rapidly generates high-quality Spatial Transcriptomics libraries. Given the wide use of the Agilent Bravo Automated Liquid Handling Platform in research laboratories and facilities, this will allow many researchers to quickly create robust Spatial Transcriptomics libraries.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Bioteknologi
- Biotechnology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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