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Transient state mic...
Transient state microscopy : a new tool for biomolecular imaging
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- Sanden, Tor (författare)
- KTH,Experimentell biomolekylär fysik
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- Persson, Gustav (författare)
- KTH,Experimentell biomolekylär fysik
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- Widengren, Jerker (författare)
- KTH,Experimentell biomolekylär fysik
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(creator_code:org_t)
- Bellingham, WA : SPIE-INT SOC OPTICAL ENGINEERING, 2009
- 2009
- Engelska.
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Ingår i: MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES IX. - Bellingham, WA : SPIE-INT SOC OPTICAL ENGINEERING. - 9780819474292
- Relaterad länk:
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https://urn.kb.se/re...
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visa fler...
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https://doi.org/10.1...
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visa färre...
Abstract
Ämnesord
Stäng
- Photoinduced transient dark states are exhibited by practically all common fluorophores. However, their information content has to date only been sparsely exploited due to methodological constraints. Here, a new concept is presented and verified that can monitor and image these states via their photodynamic fingerprints. It unites the environmental sensitivity of these states with the sensitivity of fluorescence-based detection. For demonstration, triplet state images of liposomes in different environments were generated, showing how local environmental differences can be resolved, not clearly distinguishable via other fluorescence parameters. The concept can provide several new, useful and independent fluorescence-based parameters in biomolecular imaging.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- modulated excitation
- laser scanning microscopy
- transient state imaging
- triplet state imaging
- oxygen monitoring
- Analytical chemistry
- Analytisk kemi
Publikations- och innehållstyp
- ref (ämneskategori)
- kon (ämneskategori)
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