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A multi-scale map o...
A multi-scale map of cell structure fusing protein images and interactions
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- Qin, Yue (author)
- Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA.;Univ Calif San Diego, Bioinformat & Syst Biol Program, La Jolla, CA 92093 USA.
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- Huttlin, Edward L. (author)
- Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USA.
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- Winsnes, Casper F. (author)
- KTH,Science for Life Laboratory, SciLifeLab
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- Gosztyla, Maya L. (author)
- Univ Calif San Diego, Dept Cellular & Mol Med, La Jolla, CA 92093 USA.;Univ Calif San Diego, Stem Cell Program, La Jolla, CA 92093 USA.;Univ Calif San Diego, Inst Genom Med, La Jolla, CA 92093 USA.
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- Wacheul, Ludivine (author)
- Univ Libre Bruxelles ULB, RNA Mol Biol, Fonds Rech Sci FRS FNRS, Gosselies, Belgium.
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- Kelly, Marcus R. (author)
- Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA.
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- Blue, Steven M. (author)
- Univ Calif San Diego, Dept Cellular & Mol Med, La Jolla, CA 92093 USA.;Univ Calif San Diego, Stem Cell Program, La Jolla, CA 92093 USA.;Univ Calif San Diego, Inst Genom Med, La Jolla, CA 92093 USA.
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- Zheng, Fan (author)
- Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA.
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- Chen, Michael (author)
- Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA.
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- Schaffer, Leah, V (author)
- Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA.
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- Licon, Katherine (author)
- Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA.
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- Bäckström, Anna (author)
- KTH,Science for Life Laboratory, SciLifeLab
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- Vaites, Laura Pontano (author)
- Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USA.
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- Lee, John J. (author)
- Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA.
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- Ouyang, Wei (author)
- KTH,Science for Life Laboratory, SciLifeLab
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- Liu, Sophie N. (author)
- Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA.
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- Zhang, Tian (author)
- Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USA.
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- Silva, Erica (author)
- Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA.
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- Park, Jisoo (author)
- Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA.
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- Pitea, Adriana (author)
- Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA.
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- Kreisberg, Jason F. (author)
- Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA.
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- Gygi, Steven P. (author)
- Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USA.
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- Ma, Jianzhu (author)
- Peking Univ, Inst Artificial Intelligence, Beijing, Peoples R China.
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- Harper, J. Wade (author)
- Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USA.
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- Yeo, Gene W. (author)
- Univ Calif San Diego, Bioinformat & Syst Biol Program, La Jolla, CA 92093 USA.;Univ Calif San Diego, Dept Cellular & Mol Med, La Jolla, CA 92093 USA.;Univ Calif San Diego, Stem Cell Program, La Jolla, CA 92093 USA.;Univ Calif San Diego, Inst Genom Med, La Jolla, CA 92093 USA.
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- Lafontaine, Denis L. J. (author)
- Univ Libre Bruxelles ULB, RNA Mol Biol, Fonds Rech Sci FRS FNRS, Gosselies, Belgium.
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- Lundberg, Emma (author)
- KTH,Science for Life Laboratory, SciLifeLab,;Stanford Univ, Dept Genet, Stanford, CA 94305 USA.;Chan Zuckerberg Biohub, San Francisco, CA 94158 USA.
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- Ideker, Trey (author)
- Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA.;Univ Calif San Diego, Bioinformat & Syst Biol Program, La Jolla, CA 92093 USA.;Univ Calif San Diego, Inst Genom Med, La Jolla, CA 92093 USA.;Univ Calif San Diego, Dept Comp Sci & Engn, La Jolla, CA 92093 USA.;Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA.
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Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA;Univ Calif San Diego, Bioinformat & Syst Biol Program, La Jolla, CA 92093 USA. Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USA. (creator_code:org_t)
- 2021-11-24
- 2021
- English.
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In: Nature. - : Springer Nature. - 0028-0836 .- 1476-4687. ; 600:7889, s. 536-
- Related links:
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https://dipot.ulb.ac...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The cell is a multi-scale structure with modular organization across at least four orders of magnitude(1). Two central approaches for mapping this structure-protein fluorescent imaging and protein biophysical association-each generate extensive datasets, but of distinct qualities and resolutions that are typically treated separately(2,3). Here we integrate immunofluorescence images in the Human Protein Atlas(4) with affinity purifications in BioPlex(5) to create a unified hierarchical map of human cell architecture. Integration is achieved by configuring each approach as a general measure of protein distance, then calibrating the two measures using machine learning. The map, known as the multi-scale integrated cell (MuSIC 1.0), resolves 69 subcellular systems, of which approximately half are to our knowledge undocumented. Accordingly, we perform 134 additional affinity purifications and validate subunit associations for the majority of systems. The map reveals a pre-ribosomal RNA processing assembly and accessory factors, which we show govern rRNA maturation, and functional roles for SRRM1 and FAM120C in chromatin and RPS3A in splicing. By integration across scales, MuSIC increases the resolution of imaging while giving protein interactions a spatial dimension, paving the way to incorporate diverse types of data in proteome-wide cell maps.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Medicinsk bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Medical Biotechnology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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Nature
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To the university's database
- By the author/editor
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Qin, Yue
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Huttlin, Edward ...
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Winsnes, Casper ...
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Gosztyla, Maya L ...
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Wacheul, Ludivin ...
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Kelly, Marcus R.
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show more...
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Blue, Steven M.
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Zheng, Fan
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Chen, Michael
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Schaffer, Leah, ...
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Licon, Katherine
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Bäckström, Anna
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Vaites, Laura Po ...
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Lee, John J.
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Ouyang, Wei
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Liu, Sophie N.
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Zhang, Tian
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Silva, Erica
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Park, Jisoo
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Pitea, Adriana
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Kreisberg, Jason ...
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Gygi, Steven P.
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Ma, Jianzhu
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Harper, J. Wade
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Yeo, Gene W.
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Lafontaine, Deni ...
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Lundberg, Emma
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Ideker, Trey
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- About the subject
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
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and Medical Biotechn ...
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Nature
- By the university
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Royal Institute of Technology