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Sökning: onr:"swepub:oai:DiVA.org:kth-322569" > Structural Properti...

  • Butina, KarenKTH,Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES,Department of Neuroscience, Karolinska Institutet, Solnavägen 9, Stockholm, 171 77, Sweden,Karolinska Inst, Sweden; KTH Royal Inst Technol, Sweden; Karolinska Inst, Sweden (författare)

Structural Properties Dictating Selective Optotracer Detection of Staphylococcus aureus

  • Artikel/kapitelEngelska2022

Förlag, utgivningsår, omfång ...

  • 2022-04
  • Wiley,2022
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:kth-322569
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-322569URI
  • https://doi.org/10.1002/cbic.202100684DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-184429URI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:149215070URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • QC 20230321
  • Funding Agencies|AIMES -Center for the Advancement of Integrated Medical and Engineering Sciences at Karolinska Institutet [1-249/2019]; KTH Royal Institute of Technology [VF-2019-0110]; Erling-Persson Family Foundation [4-1784/2016]; Swedish Research CouncilSwedish Research CouncilEuropean Commission [00748]; Swedish Foundation for Strategic ResearchSwedish Foundation for Strategic Research [SB12-00347]; Getinge AB [4-1599/2018]
  • Optotracers are conformation-sensitive fluorescent tracer molecules that detect peptide- and carbohydrate-based biopolymers. Their binding to bacterial cell walls allows selective detection and visualisation of Staphylococcus aureus (S. aureus). Here, we investigated the structural properties providing optimal detection of S. aureus. We quantified spectral shifts and fluorescence intensity in mixes of bacteria and optotracers, using automatic peak analysis, cross-correlation, and area-under-curve analysis. We found that the length of the conjugated backbone and the number of charged groups, but not their distribution, are important factors for selective detection of S. aureus. The photophysical properties of optotracers were greatly improved by incorporating a donor-acceptor-donor (D-A-D)-type motif in the conjugated backbone. With significantly reduced background and binding-induced on-switch of fluorescence, these optotracers enabled real-time recordings of S. aureus growth. Collectively, this demonstrates that chemical structure and photophysics are key tunable characteristics in the development of optotracers for selective detection of bacterial species. 

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Lantz, Linda,1985-Linköpings universitet,Kemi,Tekniska fakulteten(Swepub:liu)linla85 (författare)
  • Choong, Ferdinand X.Karolinska Institutet,KTH,Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES,Department of Neuroscience, Karolinska Institutet, Solnavägen 9, Stockholm, 171 77, Sweden,Karolinska Inst, Sweden; KTH Royal Inst Technol, Sweden; Karolinska Inst, Sweden(Swepub:kth)PI000000 (författare)
  • Tomac, AnaKTH,Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES,Department of Neuroscience, Karolinska Institutet, Solnavägen 9, Stockholm, 171 77, Sweden,Karolinska Inst, Sweden; KTH Royal Inst Technol, Sweden; Karolinska Inst, Sweden(Swepub:kth)PI000000 (författare)
  • Shirani, Hamid,1967-Linköpings universitet,Kemi,Tekniska fakulteten(Swepub:liu)hamsh43 (författare)
  • Löffler, SusanneKarolinska Institutet(Swepub:kth)PI000000 (författare)
  • Nilsson, Peter,1970-Linköpings universitet,Kemi,Tekniska fakulteten,Karolinska Inst, Sweden; KTH Royal Inst Technol, Sweden(Swepub:liu)petni61 (författare)
  • Richter-Dahlfors, AgnetaKarolinska Institutet,KTH,Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES,Department of Neuroscience, Karolinska Institutet, Solnavägen 9, Stockholm, 171 77, Sweden,Karolinska Inst, Sweden; KTH Royal Inst Technol, Sweden; Karolinska Inst, Sweden(Swepub:kth)PI000000 (författare)
  • KTHCenter for the Advancement of Integrated Medical and Engineering Sciences, AIMES (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:ChemBioChem: Wiley23:111439-42271439-7633

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