Sökning: onr:"swepub:oai:DiVA.org:kth-335096" > Ovarian ERβ cistrom...
Fältnamn | Indikatorer | Metadata |
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000 | 03154nam a2200325 4500 | |
001 | oai:DiVA.org:kth-335096 | |
003 | SwePub | |
008 | 230907| | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3350962 URI |
040 | a (SwePub)kth | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a vet2 swepub-contenttype |
072 | 7 | a ovr2 swepub-publicationtype |
100 | 1 | a Birgersson, Madeleineu KTH,Proteinvetenskap4 aut0 (Swepub:kth)u1cwmzu4 |
245 | 1 0 | a Ovarian ERβ cistrome and transcriptome reveal chromatin interaction with LRH-1 |
338 | a print2 rdacarrier | |
500 | a QC 20230908 | |
520 | a Background: Estrogen receptor beta (ERβ, Esr2) plays a pivotal role in folliculogenesis and ovulation, yet its exact mechanism of action is mainly uncharacterized.Results: We here performed ChIP-sequencing of mouse ovaries followed by complementary RNA-sequencing of wild-type and ERβ knockout ovaries. By integrating the cistrome and transcriptome, we identify its direct target genes and enriched biological functions in the ovary. This demonstrates a strong impact on genes regulating organism development, cell migration, lipid metabolism, response to hypoxia, and response to estrogen. Cell-type deconvolution analysis of the bulk RNA-seq data revealed a decrease in luteal cells and an increased proportion of theca cells and a specific type of cumulus cells upon ERβ loss. Moreover, we identify a significant overlap with the gene regulatory network of liver receptor homolog 1 (LRH-1). ERβ and LRH-1 extensively bind to the same chromatin locations in granulosa cells and we corroborate simultaneous co-binding using ChIP re-ChIP, at the ERβ-repressed gene Greb1. At other shared sites (by ERβ-upregulated genes Cyp11a1 and Fkbp5), they do not bind simultaneously. Transactivation assay experimentation further show that ERβ and LRH-1 can inhibit their respective transcriptional activity at classical response elements.Conclusions: We characterize genome-wide ERβ chromatin binding and gene regulations which reveal extensive crosstalk between ERβ and LRH-1. We experimentally corroborate co-binding to target genes and impact on transactivation. Our data offer genome-wide mechanistic underpinnings of ovarian physiology and fertility. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Medicinsk bioteknologi0 (SwePub)304012 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Medical Biotechnology0 (SwePub)304012 hsv//eng |
700 | 1 | a Indukuri, Rajithau KTH,Cellulär och klinisk proteomik4 aut0 (Swepub:kth)u1h2gbg8 |
700 | 1 | a Lindquist, Linnea4 aut |
700 | 1 | a Stepanauskaite, Linau KTH,Cellulär och klinisk proteomik4 aut0 (Swepub:kth)u1xd7zej |
700 | 1 | a Luo, Qing4 aut |
700 | 1 | a Deng, Qiaolin4 aut |
700 | 1 | a Archer, Amenau KTH,Cellulär och klinisk proteomik4 aut0 (Swepub:kth)u131bapq |
700 | 1 | a Williams, Cecilia,c Professor,d 1969-u KTH,Cellulär och klinisk proteomik4 aut0 (Swepub:kth)u1ygqmuy |
710 | 2 | a KTHb Proteinvetenskap4 org |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-335096 |
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