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  • Sancho, S.University of Girona, Campus de Montilivi, s/n, 17071 Girona, Spain (author)

Effects of cryopreservation on semen quality and the expression of sperm membrane hexose transporters in the spermatozoa of Iberian pigs

  • Article/chapterEnglish2007

Publisher, publication year, extent ...

  • BioScientifica,2007
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:liu-101776
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-101776URI
  • https://doi.org/10.1530/REP-07-0118DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • This study evaluated the effects of cooling, freezing and thawing on the plasma membrane integrity, kinetics and expression of two sugar transporters glucose transporter-3 and -5 (GLUT-3 and GLUT-5) in spermatozoa from Iberian boars. Semen samples were collected twice weekly from eight young, fertile Iberian boars of the Entrepelado and Lampino breeds. The samples were suspended in a commercial extender and refrigerated to 17 degrees C for transport to the laboratory (step A), where they were further extended with a lactose-egg yolk-based extender and chilled to 5 degrees C (step B) prior to freezing in the presence of glycerol (3%). Spermatozoa were assessed for plasma membrane integrity and sperm motility at each of the steps, including post-thaw (step C). Aliquots were also prepared for immunocytochemical localisation of the sugar transporters (fixed and thin smears for transmission and scanning electron microscopy levels respectively) and for SDS-PAGE electrophoresis and subsequent western blotting, using the same antibodies (rabbit anti-GLUT-3 and anti-GLUT-5 polyclonal antibodies). The results showed lower percentages of progressively motile spermatozoa at step C in both breeds, while the percentage of live spermatozoa was significantly lower only in the Entrepelado breed. The results obtained from electron microscopy clearly showed that Iberian boar spermatozoa expressed the hexose transporters, GLUT-3 and GLUT-5. The pattern of expression, in terms of location and concentration, was characteristic in each case but, in the case of isoform GLUT-5, it remained constant during the different steps of freezing-thawing protocol. These results indicate that cryopreservation affects the status of sperm cells of Iberian boars by altering the distribution of some membrane receptors and decreasing the percentage values of parameters linked to sperm quality.

Subject headings and genre

  • TECHNOLOGY
  • TEKNIKVETENSKAP

Added entries (persons, corporate bodies, meetings, titles ...)

  • Casas, I.University of Girona, Campus de Montilivi, s/n, 17071 Girona, Spain (author)
  • Ekwal, H.Swedish University of Agricultural Sciences (SLU), SE-750 07, Uppsala, Sweden (author)
  • Saravia, F.Swedish University of Agricultural Sciences (SLU), SE-750 07, Uppsala, Sweden (author)
  • Rodriguez-Martinez, HeribertoSwedish University of Agricultural Sciences (SLU), SE-750 07, Uppsala, Sweden(Swepub:liu)herro55 (author)
  • E. Rodriguez-Gil, J.Autonomous University of Barcelona, Barcelona E-08193, Spain (author)
  • Flores, E.Autonomous University of Barcelona, Barcelona E-08193, Spain (author)
  • Pinart, E.University of Girona, Campus de Montilivi, s/n, 17071 Girona, Spain (author)
  • Briz, M.University of Girona, Campus de Montilivi, s/n, 17071 Girona, Spain (author)
  • Garcia-Gil, N.University of Girona, Campus de Montilivi, s/n, 17071 Girona, Spain (author)
  • Bassols, J.University of Girona, Campus de Montilivi, s/n, 17071 Girona, Spain (author)
  • Pruneda, A.University of Girona, Campus de Montilivi, s/n, 17071 Girona, Spain (author)
  • Bussalleu, E.University of Girona, Campus de Montilivi, s/n, 17071 Girona, Spain (author)
  • Yeste, M.University of Girona, Campus de Montilivi, s/n, 17071 Girona, Spain (author)
  • Bonet, S.University of Girona, Campus de Montilivi, s/n, 17071 Girona, Spain (author)
  • University of Girona, Campus de Montilivi, s/n, 17071 Girona, SpainSwedish University of Agricultural Sciences (SLU), SE-750 07, Uppsala, Sweden (creator_code:org_t)

Related titles

  • In:Reproduction: BioScientifica134:1, s. 111-1211470-16261476-3990

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