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Influence of semina...
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Caballero, IUniversity of Murcia, Spain;
(författare)
Influence of seminal plasma PSP-I/PSP-II spermadhesin on pig gamete interaction
- Artikel/kapitelEngelska2005
Förlag, utgivningsår, omfång ...
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Cambridge University Press (CUP),2005
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-101822
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-101822URI
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https://doi.org/10.1017/S0967199405003072DOI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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The seminal plasma PSP-I/PSP-II spermadhesin is able to preserve, in vitro, the viability of highly extended boar spermatozoa, suggesting it might be used as a suitable ameliorator for the damaging effects of sperm handling, including in vitro fertilization. However, little is known about the ligand capability of PSP-I/PSP-II as regards the zona pellucida (ZP) or its possible role in gamete interaction. The present study evaluated the effect of the presence of PSP-I/PSP-II (1.5 mg/ml) during in vitro oocyte maturation and also during co-incubation of frozen-thawed boar spermatozoa with either immature (IM) or in vitro matured (IVM) oocytes, either enclosed by cumulus cells or denuded. Exposure of the gametes to the heterodimer during in vitro gamete co-incubation showed a significant blocking effect of sperm penetration rates and a decreased number of spermatozoa per oocyte in both IM and IVM denuded oocytes. Such an effect was not present in cumulus-enclosed oocytes, suggesting the effect could be mediated by exposed ZP receptors. In addition, when PSP-I/PSP-II was added to the IVM medium, oocyte maturation rates were significantly reduced. In conclusion, the results suggest that PSP-I/PSP-II, when present in vitro, blocks sperm-ZP binding.
Ämnesord och genrebeteckningar
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gametes; IVF; pig; PSP-I/PSP-II; seminal plasma
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TECHNOLOGY
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TEKNIKVETENSKAP
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Vazquez, JMUniversity of Murcia, Spain;
(författare)
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Rodriguez-Martinez, HeribertoUniversity of Murcia, Spain;(Swepub:liu)herro55
(författare)
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Gil, MAUniversity of Murcia, Spain;
(författare)
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Calvete, JJUniversity of Murcia, Spain;
(författare)
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Sanz, LInstitute of Biomedicine, CSIC, Valencia, Spain;
(författare)
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Garcia, EMInstitute of Biomedicine, CSIC, Valencia, Spain;
(författare)
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Roca, JUniversity of Murcia, Spain;
(författare)
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Martinez, EAUniversity of Murcia, Spain;
(författare)
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University of Murcia, Spain;Institute of Biomedicine, CSIC, Valencia, Spain;
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Zygote (Cambridge. Print): Cambridge University Press (CUP)13:1, s. 11-160967-19941469-8730
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