Sökning: onr:"swepub:oai:DiVA.org:liu-130198" > Probes and techniqu...
Fältnamn | Indikatorer | Metadata |
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000 | 03103naa a2200361 4500 | |
001 | oai:DiVA.org:liu-130198 | |
003 | SwePub | |
008 | 160714s2010 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-1301982 URI |
024 | 7 | a https://doi.org/10.1111/j.1439-0531.2010.01622.x2 DOI |
040 | a (SwePub)liu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Martínez-Pastor, Felipeu ITRA-ULE, INDEGSAL, Departments of Molecular Biology (Cell Biology), University of León, León, Spain4 aut |
245 | 1 0 | a Probes and techniques for sperm evaluation by flow cytometry |
264 | c 2010-05-17 | |
264 | 1 | b Blackwell Verlag,c 2010 |
338 | a print2 rdacarrier | |
520 | a CONTENTS: Flow cytometry has become an important technique in sperm evaluation and is increasingly used both for routine assessment and for research in veterinary science. We have revised the literature, describing fluorescent probes that have been used for analysing spermatozoa by flow cytometry, regarding: viability, acrosomal status, capacitation, mitochondrial status, apoptotic markers, oxidative stress markers, DNA damage, sperm counting and sperm sizing. Details and problems of some techniques are reviewed, with special attention to the occurrence of non-sperm particles in the samples ('debris'). New and promising aspects of flow cytometry, such as sperm sorting using viability markers as selection criteria, are highlighted. The relationship between flow cytometry analyses and fertility and their future improvements are considered. | |
650 | 7 | a NATURVETENSKAPx Biologix Utvecklingsbiologi0 (SwePub)106142 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Developmental Biology0 (SwePub)106142 hsv//eng |
700 | 1 | a Mata-Campuzano, Mariau ITRA-ULE, INDEGSAL, Departments of Molecular Biology (Cell Biology), University of León, León, Spain4 aut |
700 | 1 | a Alvarez-Rodriguez, Manuelu ITRA-ULE, INDEGSAL, Animal Reproduction and Obstetrics, University of León, León, Spain4 aut0 (Swepub:liu)manal80 |
700 | 1 | a Alvarez, Mercedesu ITRA-ULE, INDEGSAL, Animal Reproduction and Obstetrics, University of León, León, Spain4 aut |
700 | 1 | a Anel, Luisu ITRA-ULE, INDEGSAL, Animal Reproduction and Obstetrics, University of León, León, Spain4 aut |
700 | 1 | a de Paz, Paulinou ITRA-ULE, INDEGSAL, Departments of Molecular Biology (Cell Biology), University of León, León, Spain4 aut |
710 | 2 | a ITRA-ULE, INDEGSAL, Departments of Molecular Biology (Cell Biology), University of León, León, Spainb ITRA-ULE, INDEGSAL, Animal Reproduction and Obstetrics, University of León, León, Spain4 org |
773 | 0 | t Reproduction in domestic animalsd : Blackwell Verlagg 45 Suppl 2, s. 67-78q 45 Suppl 2<67-78x 0936-6768x 1439-0531 |
856 | 4 | u https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1439-0531.2010.01622.x |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-130198 |
856 | 4 8 | u https://doi.org/10.1111/j.1439-0531.2010.01622.x |
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