Search: onr:"swepub:oai:DiVA.org:liu-136850" >
An integrated dual ...
An integrated dual functional recognition/amplification bio-label for the one-step impedimetric detection of Micro-RNA-21
-
- Azzouzi, Sawsen (author)
- Linköpings universitet,Biosensorer och bioelektronik,Tekniska fakulteten,University of Sousse, Tunisia
-
- Mak, Wing Cheung (author)
- Linköpings universitet,Biosensorer och bioelektronik,Tekniska fakulteten
-
- Kor, Kamalodin (author)
- Linköpings universitet,Biosensorer och bioelektronik,Tekniska fakulteten,Iranian National Institute Oceanog and Atmospher Science, Iran
-
show more...
-
- Turner, Anthony (author)
- Linköpings universitet,Biosensorer och bioelektronik,Tekniska fakulteten
-
- Ben Ali, Mounir (author)
- University of Sousse, Higher Institute of Applied Sciences and Technology of Sousse, GREENS-ISSAT, Cité Ettafala, 4003 Ibn Khaldoun Sousse, Tunisia
-
- Beni, Valerio (author)
- Linköpings universitet,Institutionen för fysik, kemi och biologi,Tekniska fakulteten,ACREO SWEDISH ICE AB, Sweden
-
show less...
-
(creator_code:org_t)
- ELSEVIER ADVANCED TECHNOLOGY, 2017
- 2017
- English.
-
In: Biosensors & bioelectronics. - : ELSEVIER ADVANCED TECHNOLOGY. - 0956-5663 .- 1873-4235. ; 92, s. 154-161
- Related links:
-
https://liu.diva-por... (primary) (Raw object)
-
show more...
-
http://liu.diva-port...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Alteration in expression of miRNAs has been correlated with different cancer types, tumour stage and response to treatments. In this context, a structurally responsive oligonucleotide-based electrochemical impedimetric biosensor has been developed for the simple and sensitive detection of miRNA-21. A highly specific biotinylated DNA/LNA molecular beacon (MB) probe was conjugated with gold nanoparticles (AuNPs) to create an integrated, dual function bio-label (biotin-MB-AuNPs) for both biorecognition and signal generation. In the presence of target miRNA-21, hybridisation takes place resulting in the "activation" of the biotin-MB; this event makes the biotin group, which was previously "protected" by the steric hindrance of the MB stem-loop structure, accessible. The activated biotin-MB-AuNPs/miRNA complexes become available for capture, via supramolecular interaction, onto a nentravidin-modified electrode for electrochemical transduction. The binding event results in a decrease of the charge transfer resistance at the working electrode/electrolyte interface. The biosensor responded linearly in the range 1-1000 pM of miRNA-21, with a limit of detection of 0.3 pM, good reproducibility (Relative Standard deviation (RSD) =3.3%) and high selectivity over other miRNAs (i.e. miRNA221 and miRNA-205) sequences. Detection of miRNA-21 in spiked serum samples at clinically relevant levels (low pM range) was also demonstrated, thus illustrating the potential of the biosensor for point-of-care clinical applications. The proposed biosensor design, based on the combination of a neutravidin transducing surface and the dual-function biotin-MB-AuNPs bio-label, provides a simple and robust approach for detection of short-length nucleic acid targets, such as miRNAs.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- MicroRNA-21; Gold nanoparticles; Molecular beacon; Electrochemical impedance spectroscopy
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database