SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:DiVA.org:liu-164614"
 

Search: onr:"swepub:oai:DiVA.org:liu-164614" > 16S rRNAgene-based ...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

16S rRNAgene-based metagenomic analysis of the gut microbial community associated with the DUI species Unio crassus (Bivalvia: Unionidae)

Mioduchowska, Monika (author)
Univ Gdansk, Poland
Zajac, Katarzyna (author)
Polish Acad Sci, Poland
Bartoszek, Krzysztof (author)
Linköpings universitet,Statistik och maskininlärning,Filosofiska fakulteten
show more...
Madanecki, Piotr (author)
Med Univ Gdansk, Poland
Kur, Jaroslaw (author)
Empty Spaces Res, Poland
Zajac, Tadeusz (author)
Polish Acad Sci, Poland
show less...
 (creator_code:org_t)
2020-03-02
2020
English.
In: Journal of Zoological Systematics and Evolutionary Research. - : WILEY. - 0947-5745 .- 1439-0469. ; 58:2, s. 615-623
  • Journal article (peer-reviewed)
Abstract Subject headings
Close  
  • What factors determine biome richness: genetic or environmental? Sex, phylogeny, and tolerance indicated by other symbionts (e.g., endosymbionts) or simply is it related to local habitat, especially if the gut biome is considered? To answer these questions, we investigated the gut microbial profile of both sexes of three Unio crassus populations, species with unique system of mitochondrial DNA inheritance called doubly uniparental inheritance (DUI), living in different ecological conditions. High-throughput sequencing of the V3-V4 hypervariable regions in the bacterial 16S rRNA gene fragment was performed, which resulted in a total of 1,051,647 reads, with 58,424 reads/65 OTUs (operational taxonomic units) per sample on average. We identified a core microbiome, with all individual mussels sharing 69 OTUs (representing 23% of the total number of OTUs). Proteobacteria was the dominant phylum in all samples, followed by Firmicutes, Actinobacteria, and Bacteroidetes. There were no significant differences in gut microbiome compositions between the two sexes of this species; however, we observed different phyla in geographically isolated populations. A non-metric multidimensional scaling plot and dendrogram showed that the bacterial profile complies with the genetic structure of populations. Although we found differences in microbiomes between populations, their genetic structure suggests that the microbiome is weakly related to habitat, and more strongly to phylogeography (on both F and M mitotypes). We found no significant differences in beta diversity between the individuals of the bacterial communities measured using the Bray-Curtis index. Finally, we also examined whether OTUs were represented by symbiotic bacteria that enable cellulose digestion and by endosymbiotic bacteria that play important functions in the biology of their hosts and also affect microevolutionary processes and population phenomena. With regard to the endosymbionts, however, there was no relation to sex of the studied individuals, which suggests that there are no straightforward relations between DUI and microbiome.

Subject headings

NATURVETENSKAP  -- Biologi -- Ekologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Ecology (hsv//eng)

Keyword

freshwater mussels; gut microbiome; next-generation sequencing; operational taxonomic units (OTUs) assignment; Unionidae

Publication and Content Type

ref (subject category)
art (subject category)

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Search outside SwePub

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view