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A Low Cost Antibody...
A Low Cost Antibody Signal Enhancer Improves Immunolabeling in Cell Culture, Primate Brain and Human Cancer Biopsy
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- Flores-Maldonado, Catalina (författare)
- IPN, Mexico
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- Estela Albino-Sanchez, M. (författare)
- IPN, Mexico
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- Rodriguez-Callejas, Juan D. (författare)
- IPN, Mexico
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- Estrada-Mondragon, Argel (författare)
- Linköpings universitet,Avdelningen för neurobiologi,Medicinska fakulteten
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- Leon-Galicia, Ismael (författare)
- Univ Nacl Autonoma Mexico, Mexico; Secretaria Salud Mexico, Mexico
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- Maqueda-Alfaro, Raul (författare)
- IPN, Mexico
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- Perez-Cruz, Claudia (författare)
- IPN, Mexico
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- Fuchs, Eberhard (författare)
- Leibniz Inst Primate Res, Germany
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- Garcia-Carranca, Alejandro (författare)
- Univ Nacl Autonoma Mexico, Mexico; Secretaria Salud Mexico, Mexico
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- Contreras, Ruben G. (författare)
- IPN, Mexico
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- Missirlis, Fanis (författare)
- IPN, Mexico
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- Rosas-Arellano, Abraham (författare)
- Univ Nacl Autonoma Mexico, Mexico
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(creator_code:org_t)
- Elsevier, 2020
- 2020
- Engelska.
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Ingår i: Neuroscience. - : Elsevier. - 0306-4522 .- 1873-7544. ; 439, s. 275-286
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https://liu.diva-por... (primary) (Raw object)
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http://liu.diva-port...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The use of antibodies to identify neuronal receptors, neurotransmitters, cytoskeletal elements or pathologic protein aggregates, ion channels, adhesion molecules or other cell-type specific markers, is common practice in neuroscience. Antibody detection systems are often based on confocal, epifluorescence or brightfield microscopy. Three types of technical issues can interfere with immunolabeling: low abundance of the target protein, low specific affinity of the antibody and/or signal background sometimes related to tissue fixation. Here, giving tribute to Professor Miledis mentorship, we propose the application of an antibody signal enhancer (ASE) solution based on glycine, hydrogen peroxide and a detergent mix as a simple, low cost, protocol variation that significantly and specifically improves the signal to noise ratio during immunostaining experiments. We describe three new settings in which ASE improves the detection of a variety of antibodies applied on long-time stored non-human primate brain sections, cell culture monolayers and on squamous carcinomas retrieved from cervical cancer patients. The significant improvement of ASE over optimized immunohistochemical protocols used in clinical practice (i.e. cancer detection) combined with its simplicity and low cost makes it an attractive method for biomedical applications. This article is part of a Special Issue entitled: Honoring Ricardo Miledi - outstanding neuroscientist of XX-XXI centuries. (C) 2020 IBRO. Published by Elsevier Ltd. All rights reserved.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- astrocytes; claudins; early diagnosis; EGF; GFAP; human cervix; Ki67; Lgr5; biomedical application; Sox2
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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