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Protocol for cross-...
Protocol for cross-platform characterization of human and murine extracellular vesicles and particles
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- Bojmar, Linda (author)
- Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten,Weill Cornell Med, NY 10065 USA
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- Kim, Han Sang (author)
- Weill Cornell Med, NY 10065 USA; Yonsei Univ, South Korea
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- Sugiura, Kei (author)
- Univ Tokyo, Japan; Tokyo Inst Technol, Japan
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- Heissel, Soren (author)
- Rockefeller Univ, NY USA
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- Lucotti, Serena (author)
- Weill Cornell Med, NY 10065 USA
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- Cioffi, Michele (author)
- Weill Cornell Med, NY 10065 USA
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- Johnson, Kofi Ennu (author)
- Weill Cornell Med, NY 10065 USA; Triinst PhD Program Computat Biol & Med, NY USA
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- Cohen-Gould, Leona (author)
- Weill Cornell Med, NY USA
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- Zhang, Haiying (author)
- Weill Cornell Med, NY 10065 USA
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- Molina, Henrik (author)
- Rockefeller Univ, NY USA
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- Matei, Irina R. (author)
- Weill Cornell Med, NY 10065 USA
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- Lyden, David (author)
- Weill Cornell Med, NY 10065 USA
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- Hoshino, Ayuko (author)
- Weill Cornell Med, NY 10065 USA; Univ Tokyo, Japan; Tokyo Inst Technol, Japan
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(creator_code:org_t)
- ELSEVIER, 2024
- 2024
- English.
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In: STAR PROTOCOLS. - : ELSEVIER. - 2666-1667. ; 5:1
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Characterization of isolated extracellular vesicles and particles (EVPs) is crucial for determining functions and biomarker potential. Here, we present a protocol to analyze size, number, morphology, and EVP protein cargo and to validate EVP proteins in both humans and mice. We describe steps for nanoparticle tracking analysis, transmission electron microscopy, single-EVP immunodetection, EVP proteomic mass spectrometry and bioinformatic analysis, and EVP protein validation by ExoELISA and western blot analysis. This allows for EVP cross -validation across different platforms.For complete details on the use and execution of this protocol, please refer to Hoshino et al.1
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Bojmar, Linda
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Kim, Han Sang
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Sugiura, Kei
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Heissel, Soren
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Lucotti, Serena
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Cioffi, Michele
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show more...
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Johnson, Kofi En ...
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Cohen-Gould, Leo ...
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Zhang, Haiying
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Molina, Henrik
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Matei, Irina R.
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Lyden, David
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Hoshino, Ayuko
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Analytical Chemi ...
- Articles in the publication
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STAR PROTOCOLS
- By the university
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Linköping University