Radiotherapy response in oral squamous carcinoma cell lines: Evaluation of apoptotic proteins as prognostic factors

Roberg, Karin,1957-Linköpings universitet,Oto-Rhino-Laryngologi,Hälsouniversitetet (författare)

Radiotherapy response in oral squamous carcinoma cell lines : Evaluation of apoptotic proteins as prognostic factors

Background. In this study, we investigated the importance of apoptosis for cell death after radiotherapy, and whether the expression of pro- and anti-apoptotic proteins has any correlation to the radiosensitivity.Methods. Three oral squamous cell carcinoma cell lines, UT-SCC-2, UT-SCC-9 and UT-SCC-24A, were subjected to radiotherapy. After irradiation, viable and dead cells were counted to determine radiation sensitivity and apoptosis was analyzed by measurement of caspase-3 activity. The expressions of pro- and anti-apoptotic proteins were assessed using western blot analyses.Results and Conclusion. After irradiation, apoptotic morphology and caspase-3 activity were only detected in cell lines exhibiting high or moderate radiosensitivity. Western blot analysis indicates that survivin, epidermal growth factor receptor, cyclooxygenase-2, and Bcl-xL are critical components in irradiation resistance of the investigated cell lines. Moreover, our results suggest that apoptotic cell death and the balance between pro- and anti-apoptotic proteins are of importance for the outcome of radiotherapy.

Jonsson, Ann-CharlotteLinköpings universitet,Medicinsk radiofysik,Hälsouniversitetet(Swepub:liu)annjo32 (författare)
Grénman, ReidarDepartment of Otorhinolaryngology, Head and Neck Surgery, Central Hospital and University of Turku and Medical Biochemistry, University of Turku, Finland (författare)
Norberg-Spaak, Lena,1956-Linköpings universitet,Oto-Rhino-Laryngologi,Hälsouniversitetet(Swepub:liu)lenno72 (författare)
Linköpings universitetOto-Rhino-Laryngologi (creator_code:org_t)

Av författaren/redakt...: Roberg, Karin, 1 ...; Jonsson, Ann-Cha ...; Grénman, Reidar; Norberg-Spaak, L ...

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