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Continental scienti...
Continental scientific drilling and microbiology : (extremely) low biomass in bedrock of central Sweden
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- Westmeijer, George (author)
- Linnéuniversitetet,Institutionen för biologi och miljö (BOM),Ctr Ecol & Evolut Microbial Model Syst EEMiS
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- Escudero, Cristina (author)
- Univ Autonoma Madrid, Spain;Univ Tubingen, Germany
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- Bergin, Claudia (author)
- Sci Life Lab, Sweden
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- Turner, Stephanie (author)
- Linnéuniversitetet,Institutionen för biologi och miljö (BOM),Ctr Ecol & Evolut Microbial Model Syst EEMiS
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- Ståhle, Magnus (author)
- Linnéuniversitetet,Institutionen för biologi och miljö (BOM),Ctr Ecol & Evolut Microbial Model Syst EEMiS
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- Mehrshad, Maliheh (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för vatten och miljö,Department of Aquatic Sciences and Assessment
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- Leroy, Prune (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för vatten och miljö,Department of Aquatic Sciences and Assessment
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- Buck, Moritz (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för vatten och miljö,Department of Aquatic Sciences and Assessment
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- López-Hernández, Pilar, 1987- (author)
- Sveriges lantbruksuniversitet,Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Swedish University of Agricultural Sciences, Sweden,Institutionen för vatten och miljö,Department of Aquatic Sciences and Assessment,Stockholm University
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- Kallmeyer, Jens (author)
- GFZ German Res Ctr Geosci, Germany
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- Amils, Ricardo (author)
- Univ Autonoma Madrid, Spain
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- Bertilsson, Stefan (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för vatten och miljö,Department of Aquatic Sciences and Assessment
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- Dopson, Mark, 1970- (author)
- Linnéuniversitetet,Institutionen för biologi och miljö (BOM),Ctr Ecol & Evolut Microbial Model Syst EEMiS
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(creator_code:org_t)
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- Copernicus Publications, 2024
- 2024
- English.
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In: Biogeosciences. - : Copernicus Publications. - 1726-4170 .- 1726-4189. ; 21:2, s. 591-604
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https://doi.org/10.5...
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Abstract
Subject headings
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- Scientific drilling expeditions offer a unique opportunity to characterize microbial communities in the subsurface that have long been isolated from the surface. With subsurface microbial biomass being low in general, biological contamination from the drilling fluid, sample processing, or molecular work is a major concern. To address this, characterization of the contaminant populations in the drilling fluid and negative extraction controls are essential for assessing and evaluating such sequencing data. Here, rock cores down to 2250 m depth, groundwater-bearing fractures, and the drilling fluid were sampled for DNA to characterize the microbial communities using a broad genomic approach. However, even after removing potential contaminant populations present in the drilling fluid, notorious contaminants were abundant and mainly affiliated with the bacterial order Burkholderiales. These contaminant microorganisms likely originated from the reagents used for isolating DNA despite stringent quality standards during the molecular work. The detection of strictly anaerobic sulfate reducers such as Candidatus Desulforudis audaxviator suggested the presence of autochthonous deep biosphere taxa in the sequenced libraries, yet these clades represented only a minor fraction of the sequence counts ( < 0.1 %), hindering further ecological interpretations. The described methods and findings emphasize the importance of sequencing extraction controls and can support experimental design for future microbiological studies in conjunction with continental drilling operations.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- Mikrobiologi
- Microbiology
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Westmeijer, Geor ...
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Escudero, Cristi ...
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Bergin, Claudia
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Turner, Stephani ...
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Ståhle, Magnus
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Mehrshad, Malihe ...
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show more...
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Leroy, Prune
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Buck, Moritz
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López-Hernández, ...
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Kallmeyer, Jens
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Amils, Ricardo
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Bertilsson, Stef ...
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Dopson, Mark, 19 ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
- Articles in the publication
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Biogeosciences
- By the university
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Linnaeus University
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Stockholm University
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Swedish University of Agricultural Sciences