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UCYN-A3, a newly ch...
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Cornejo-Castillo, Francisco M.Univ Calif Santa Cruz, USA;CSIC, Spain
(författare)
UCYN-A3, a newly characterized open ocean sublineage of the symbiotic N2-fixing cyanobacterium Candidatus Atelocyanobacterium thalassa
- Artikel/kapitelEngelska2019
Förlag, utgivningsår, omfång ...
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2018-11-08
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Wiley-Blackwell,2019
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:lnu-80287
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https://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-80287URI
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https://doi.org/10.1111/1462-2920.14429DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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The symbiotic unicellular cyanobacterium Candidatus Atelocyanobacterium thalassa (UCYN-A) is one of the most abundant and widespread nitrogen (N-2)-fixing cyanobacteria in the ocean. Although it remains uncultivated, multiple sublineages have been detected based on partial nitrogenase (nifH) gene sequences, including the four most commonly detected sublineages UCYN-A1, UCYN-A2, UCYN-A3 and UCYN-A4. However, very little is known about UCYN-A3 beyond the nifH sequences from nifH gene diversity surveys. In this study, single cell sorting, DNA sequencing, qPCR and CARD-FISH assays revealed discrepancies involving the identification of sublineages, which led to new information on the diversity of the UCYN-A symbiosis. 16S rRNA and nifH gene sequencing on single sorted cells allowed us to identify the 16S rRNA gene of the uncharacterized UCYN-A3 sublineage. We designed new CARD-FISH probes that allowed us to distinguish and observe UCYN-A2 in a coastal location (SIO Pier; San Diego) and UCYN-A3 in an open ocean location (Station ALOHA; Hawaii). Moreover, we reconstructed about 13% of the UCYN-A3 genome from Tara Oceans metagenomic data. Finally, our findings unveil the UCYN-A3 symbiosis in open ocean waters suggesting that the different UCYN-A sublineages are distributed along different size fractions of the plankton defined by the cell-size ranges of their prymnesiophyte hosts.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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del Carmen Munoz-Marin, MariaUniv Calif Santa Cruz, USA;Univ Cordoba, Spain
(författare)
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Turk-Kubo, Kendra A.Univ Calif Santa Cruz, USA
(författare)
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Royo-Llonch, MartaCSIC, Spain
(författare)
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Farnelid, Hanna,1983-Linnéuniversitetet,Institutionen för biologi och miljö (BOM),Univ Calif Santa Cruz, USA,Ctr Ecol & Evolut Microbial Model Syst EEMiS(Swepub:lnu)efaha
(författare)
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Acinas, Silvia G.CSIC, Spain
(författare)
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Zehr, Jonathan P.Univ Calif Santa Cruz, USA
(författare)
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Univ Calif Santa Cruz, USA;CSIC, SpainUniv Calif Santa Cruz, USA;Univ Cordoba, Spain
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Environmental Microbiology: Wiley-Blackwell21:1, s. 111-1241462-29121462-2920
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