Segmented flow generator for serial crystallography at the European X-ray free electron laser

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Echelmeier, A. (author)

Segmented flow generator for serial crystallography at the European X-ray free electron laser

Article/chapterEnglish2020

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2020-09-09
Nature Research,2020
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LIBRIS-ID:oai:DiVA.org:miun-41548
https://urn.kb.se/resolve?urn=urn:nbn:se:miun:diva-41548URI
https://doi.org/10.1038/s41467-020-18156-7DOI

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Language:English
Summary in:English

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SwePubSwePub

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) allows structure determination of membrane proteins and time-resolved crystallography. Common liquid sample delivery continuously jets the protein crystal suspension into the path of the XFEL, wasting a vast amount of sample due to the pulsed nature of all current XFEL sources. The European XFEL (EuXFEL) delivers femtosecond (fs) X-ray pulses in trains spaced 100 ms apart whereas pulses within trains are currently separated by 889 ns. Therefore, continuous sample delivery via fast jets wastes >99% of sample. Here, we introduce a microfluidic device delivering crystal laden droplets segmented with an immiscible oil reducing sample waste and demonstrate droplet injection at the EuXFEL compatible with high pressure liquid delivery of an SFX experiment. While achieving ~60% reduction in sample waste, we determine the structure of the enzyme 3-deoxy-D-manno-octulosonate-8-phosphate synthase from microcrystals delivered in droplets revealing distinct structural features not previously reported.

Subject headings and genre

NATURVETENSKAP Fysik Atom- och molekylfysik och optik hsv//swe
NATURAL SCIENCES Physical Sciences Atom and Molecular Physics and Optics hsv//eng
crystallography
droplet
enzyme activity
high pressure
laser method
segmentation
water treatment
aqueous solution
Article
conformational transition
controlled study
crystal structure
high performance liquid chromatography
physical parameters
pulse rate
synchrotron radiation
three dimensional printing
X ray crystallography
devices
electron
hydrodynamics
lab on a chip
laser
ultrastructure
2-dehydro-3-deoxyphosphooctonate aldolase
Escherichia coli protein
lyase
Aldehyde-Lyases
Electrons
Escherichia coli Proteins
Lab-On-A-Chip Devices
Lasers

Added entries (persons, corporate bodies, meetings, titles ...)

Cruz Villarreal, J. (author)
Messerschmidt, M. (author)
Kim, D. (author)
Coe, J. D. (author)
Thifault, D. (author)
Botha, S. (author)
Egatz-Gomez, A. (author)
Gandhi, S. (author)
Brehm, G. (author)
Conrad, C. E. (author)
Hansen, D. T. (author)
Madsen, C. (author)
Bajt, S. (author)
Meza-Aguilar, J. D. (author)
Oberthür, D. (author)
Wiedorn, M. O. (author)
Fleckenstein, H. (author)
Mendez, D. (author)
Knoška, J. (author)
Martin-Garcia, J. M. (author)
Hu, H. (author)
Lisova, S. (author)
Allahgholi, A. (author)
Gevorkov, Y. (author)
Ayyer, K. (author)
Aplin, S. (author)
Ginn, H. M. (author)
Graafsma, H.Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany(Swepub:miun)exheigra (author)
Morgan, A. J. (author)
Greiffenberg, D. (author)
Klujev, A. (author)
Laurus, T. (author)
Poehlsen, J. (author)
Trunk, U. (author)
Mezza, D. (author)
Schmidt, B. (author)
Kuhn, M. (author)
Fromme, R. (author)
Sztuk-Dambietz, J. (author)
Raab, N. (author)
Hauf, S. (author)
Silenzi, A. (author)
Michelat, T. (author)
Xu, C. (author)
Danilevski, C. (author)
Parenti, A. (author)
Mekinda, L. (author)
Weinhausen, B. (author)
Mills, G. (author)
Vagovic, P. (author)
Kim, Y. (author)
Kirkwood, H. (author)
Bean, R. (author)
Bielecki, J. (author)
Stern, S. (author)
Giewekemeyer, K. (author)
Round, A. R. (author)
Schulz, J. (author)
Dörner, K. (author)
Grant, T. D. (author)
Mariani, V. (author)
Barty, A. (author)
Mancuso, A. P. (author)
Weierstall, U. (author)
Spence, J. C. H. (author)
Chapman, H. N. (author)
Zatsepin, N. (author)
Fromme, P. (author)
Kirian, R. A. (author)
Ros, A. (author)
Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany (creator_code:org_t)

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By the author/editor: Echelmeier, A.; Cruz Villarreal, ...; Messerschmidt, M ...; Kim, D.; Coe, J. D.; Thifault, D.; show more...; Botha, S.; Egatz-Gomez, A.; Gandhi, S.; Brehm, G.; Conrad, C. E.; Hansen, D. T.; Madsen, C.; Bajt, S.; Meza-Aguilar, J. ...; Oberthür, D.; Wiedorn, M. O.; Fleckenstein, H.; Mendez, D.; Knoška, J.; Martin-Garcia, J ...; Hu, H.; Lisova, S.; Allahgholi, A.; Gevorkov, Y.; Ayyer, K.; Aplin, S.; Ginn, H. M.; Graafsma, H.; Morgan, A. J.; Greiffenberg, D.; Klujev, A.; Laurus, T.; Poehlsen, J.; Trunk, U.; Mezza, D.; Schmidt, B.; Kuhn, M.; Fromme, R.; Sztuk-Dambietz, ...; Raab, N.; Hauf, S.; Silenzi, A.; Michelat, T.; Xu, C.; Danilevski, C.; Parenti, A.; Mekinda, L.; Weinhausen, B.; Mills, G.; Vagovic, P.; Kim, Y.; Kirkwood, H.; Bean, R.; Bielecki, J.; Stern, S.; Giewekemeyer, K.; Round, A. R.; Schulz, J.; Dörner, K.; Grant, T. D.; Mariani, V.; Barty, A.; Mancuso, A. P.; Weierstall, U.; Spence, J. C. H.; Chapman, H. N.; Zatsepin, N.; Fromme, P.; Kirian, R. A.; Ros, A.; show less...

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NATURAL SCIENCES: NATURAL SCIENCES; and Physical Science ...; and Atom and Molecul ...

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By the university: Mid Sweden University

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