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Lipocalin prostagla...
Lipocalin prostaglandin D synthase and PPARγ2 coordinate to regulate carbohydrate and lipid metabolism in vivo
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- Virtue, Sam (author)
- Metabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge, United Kingdom
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- Masoodi, Mojgan (author)
- Elsie Widdowson Laboratory, Medical Research Council Human Nutrition Research, Cambridge, United Kingdom
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- Velagapudi, Vidya (author)
- VTT Technical Research Centre of Finland, Espoo, Finland
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- Tan, Chong Yew (author)
- Metabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge, United Kingdom
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- Dale, Martin (author)
- University of Cambridge Metabolic Research Laboratories, Institute of Metabolic Science, Cambridge, United Kingdom
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- Suorti, Tapani (author)
- VTT Technical Research Centre of Finland, Espoo, Finland
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- Slawik, Marc (author)
- Department of Medicine Innenstadt, Endocrinology/Diabetes University Hospital, Munich, Germany
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- Blount, Margaret (author)
- University of Cambridge Metabolic Research Laboratories, Institute of Metabolic Science, Cambridge, United Kingdom
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- Burling, Keith (author)
- University of Cambridge Metabolic Research Laboratories, Institute of Metabolic Science, Cambridge, United Kingdom
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- Campbell, Mark (author)
- University of Cambridge Metabolic Research Laboratories, Institute of Metabolic Science, Cambridge, United Kingdom
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- Eguchi, Naomi (author)
- Osaka Bioscience Institute, Osaka, Japan
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- Medina-Gomez, Gema (author)
- Departamento de Bioquímica, Fisiología y Genética Molecular, Universidad Rey Juan Carlos, Madrid, Spain
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- Sethi, Jaswinder K (author)
- University of Cambridge Metabolic Research Laboratories, Institute of Metabolic Science, Cambridge, United Kingdom
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- Oresic, Matej, 1967- (author)
- Örebro universitet,Institutionen för medicinska vetenskaper,VTT Technical Research Centre of Finland, Espoo, Finland
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- Urade, Yoshihiro (author)
- Osaka Bioscience Institute, Osaka, Japan
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- Griffin, Julian L (author)
- Department of Biochemistry, Medical Research Council Human Nutrition Research, Cambridge, United Kingdom
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- Vidal-Puig, Antonio (author)
- University of Cambridge Metabolic Research Laboratories, Institute of Metabolic Science, Cambridge, United Kingdom
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(creator_code:org_t)
- 2012-07-05
- 2012
- English.
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In: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 7:7
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https://doi.org/10.1...
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Abstract
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- Mice lacking Peroxisome Proliferator-Activated Receptor γ2 (PPARγ2) have unexpectedly normal glucose tolerance and mild insulin resistance. Mice lacking PPARγ2 were found to have elevated levels of Lipocalin prostaglandin D synthase (L-PGDS) expression in BAT and subcutaneous white adipose tissue (WAT). To determine if induction of L-PGDS was compensating for a lack of PPARγ2, we crossed L-PGDS KO mice to PPARγ2 KO mice to generate Double Knock Out mice (DKO). Using DKO mice we demonstrated a requirement of L-PGDS for maintenance of subcutaneous WAT (scWAT) function. In scWAT, DKO mice had reduced expression of thermogenic genes, the de novo lipogenic program and the lipases ATGL and HSL. Despite the reduction in markers of lipolysis in scWAT, DKO mice had a normal metabolic rate and elevated serum FFA levels compared to L-PGDS KO alone. Analysis of intra-abdominal white adipose tissue (epididymal WAT) showed elevated expression of mRNA and protein markers of lipolysis in DKO mice, suggesting that DKO mice may become more reliant on intra-abdominal WAT to supply lipid for oxidation. This switch in depot utilisation from subcutaneous to epididymal white adipose tissue was associated with a worsening of whole organism metabolic function, with DKO mice being glucose intolerant, and having elevated serum triglyceride levels compared to any other genotype. Overall, L-PGDS and PPARγ2 coordinate to regulate carbohydrate and lipid metabolism.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
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Virtue, Sam
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Masoodi, Mojgan
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Velagapudi, Vidy ...
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Tan, Chong Yew
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Dale, Martin
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Suorti, Tapani
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Slawik, Marc
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Blount, Margaret
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Burling, Keith
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Campbell, Mark
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Eguchi, Naomi
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Medina-Gomez, Ge ...
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Sethi, Jaswinder ...
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Oresic, Matej, 1 ...
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Urade, Yoshihiro
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Griffin, Julian ...
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Vidal-Puig, Anto ...
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PLOS ONE
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Örebro University