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Anionic biopolymers...
Anionic biopolymers as blood flow sensors
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Siegel, G (författare)
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Kauschmann, M (författare)
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- Malmsten, M (författare)
- RISE,YKI – Ytkemiska institutet
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visa fler...
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Walter, A (författare)
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Buddecke, E (författare)
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visa färre...
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(creator_code:org_t)
- 1996
- 1996
- Engelska.
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Ingår i: Biosensors & bioelectronics. - 0956-5663 .- 1873-4235. ; 11, s. 281-294
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- The finding of flow-dependent vasodilatation rests on the basic observation that with an increase in blood flow the vessels become wider, with a decrease the vascular smooth muscle cells contract. Proteoheparan sulphate could be the sensor macromolecule at the endothelial cell membrane-blood interface, that reacts on the shear stress generated by the flowing blood, and that informs and regulates the vascular smooth muscle cells via a signal transduction chain. This anionic biopolyelectrolyte possesses viscoelastic and specific ion binding properties which allow a change of its configuration in dependence on shear stress and electrostatic charge density. The blood flow sensor undergoes a conformational transition from a random coil to an extended filamentous state with increasing flow, whereby Na+ ions from the blood are bound. Owing to the intramolecular elastic recoil forces of proteoheparan sulphate the slowing of a flow rate causes an entropic coiling the expulsion of Na+ ions and thus an interruption of the signal chain. Under physiological conditions, the conformation and Na+ binding proved to be extremely Ca2+-sensitive while K+ and Mg2+ ions play a minor role for the susceptibility of the sensor. Via counterion migration of the bound Na+ ions along the sensor glycosaminoglycan side chains and following Na+ passage through an unspecific ion channel in the endothelial cell membrane, the signal transduction chain leads to a membrane depolarization with Ca2+ influx into the cells. This stimulates the EDRF/NO production and release from the endothelial cells. The consequence is vasodilatation.
Nyckelord
- Anionic biopolymers
- cation binding
- ellipsometry
- flow-dependent vasodilatation
- proteoheparan sulphate
- 23Na+ NMR
- surface force studies
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