Sökning: onr:"swepub:oai:DiVA.org:sh-24381" >
Tick-borne encephal...
Tick-borne encephalitis virus sequenced directly from questing and blood-feeding ticks reveals quasispecies variance.
-
- Asghar, Naveed (författare)
- Södertörns högskola,Biologi,School of Natural Science, Technology & Environmental Studies, Södertörn University, Huddinge, Sweden
-
- Lindblom, Pontus (författare)
- Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Hälsouniversitetet
-
- Melik, Wessam (författare)
- Södertörns högskola,Biologi,School of Natural Science, Technology & Environmental Studies, Södertörn University, Huddinge, Sweden
-
visa fler...
-
- Lindqvist, Richard (författare)
- Umeå universitet,Virologi,Division of Virology, Department of Clinical Microbiology, Umeå University, Umeå, Sweden
-
- Haglund, Mats (författare)
- Department of Infectious Diseases, County Hospital, Kalmar, Sweden
-
- Forsberg, Pia (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Infektionskliniken i Östergötland,Avdelningen för mikrobiologi och molekylär medicin
-
- Överby, Anna K. (författare)
- Umeå universitet,Virologi,Division of Virology, Department of Clinical Microbiology, Umeå University, Umeå, Sweden
-
- Andreassen, Ashild (författare)
- Division of Infectious Disease Control, Department of Virology, Norwegian Institute of Public Health, Oslo, Norway
-
- Lindgren, Per-Eric (författare)
- Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Hälsouniversitetet
-
- Johansson, Magnus (författare)
- Örebro universitet,Södertörns högskola,Biologi,School of Natural Science, Technology & Environmental Studies, Södertörn University, Huddinge, Sweden; RiSC - Inflammatory Response and Infection Susceptibility Centre, Faculty of Medicine and Health, Örebro University, Örebro, Sweden,Institutionen för läkarutbildning,School of Natural Science, Technology & Environmental Studies, Södertörn University, Huddinge, Sweden / School of Health and Medical Sciences, Örebro University, Örebro, Sweden
-
visa färre...
-
(creator_code:org_t)
- 2014-07-24
- 2014
- Engelska.
-
Ingår i: PLOS ONE. - San Francisco, USA : Public Library of Science (PLoS). - 1932-6203. ; 9:7
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
http://www.ncbi.nlm....
-
https://journals.plo...
-
https://umu.diva-por... (primary) (Raw object)
-
https://liu.diva-por... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
https://urn.kb.se/re...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- The increased distribution of the tick-borne encephalitis virus (TBEV) in Scandinavia highlights the importance of characterizing novel sequences within the natural foci. In this study, two TBEV strains: the Norwegian Mandal 2009 (questing nymphs pool) and the Swedish Saringe 2009 (blood-fed nymph) were sequenced and phylogenetically characterized. Interestingly, the sequence of Mandal 2009 revealed the shorter form of the TBEV genome, similar to the highly virulent Hypr strain, within the 3' non-coding region (3'NCR). A different genomic structure was found in the 3'NCR of Saringe 2009, as in-depth analysis demonstrated TBEV variants with different lengths within the poly(A) tract. This shows that TBEV quasispecies exists in nature and indicates a putative shift in the quasispecies pool when the virus switches between invertebrate and vertebrate environments. This prompted us to further sequence and analyze the 3'NCRs of additional Scandinavian TBEV strains and control strains, Hypr and Neudoerfl. Toro 2003 and Habo 2011 contained mainly a short (A)3C(A)6 poly(A) tract. A similar pattern was observed for the human TBEV isolates 1993/783 and 1991/4944; however, one clone of 1991/4944 contained an (A)3C(A)11 poly(A) sequence, demonstrating that quasispecies with longer poly(A) could be present in human isolates. Neudoerfl has previously been reported to contain a poly(A) region, but to our surprise the re-sequenced genome contained two major quasispecies variants, both lacking the poly(A) tract. We speculate that the observed differences are important factors for the understanding of virulence, spread, and control of the TBEV.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- polyadenylic acid
- 3' non coding region
- adult
- article
- controlled study
- gene sequence
- gene structure
- gene switching
- genetic variability
- invertebrate
- Ixodes ricinus
- nonhuman
- nucleotide sequence
- nymph
- phylogeny
- Tick borne encephalitis flavivirus
- vertebrate
- virus genome
- virus strain
- virus virulence
- Medicine
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
-
PLOS ONE
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas
- Av författaren/redakt...
-
Asghar, Naveed
-
Lindblom, Pontus
-
Melik, Wessam
-
Lindqvist, Richa ...
-
Haglund, Mats
-
Forsberg, Pia
-
visa fler...
-
Överby, Anna K.
-
Andreassen, Ashi ...
-
Lindgren, Per-Er ...
-
Johansson, Magnu ...
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Infektionsmedici ...
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Mikrobiologi
- Artiklar i publikationen
-
PLOS ONE
- Av lärosätet
-
Södertörns högskola
-
Örebro universitet
-
Umeå universitet
-
Linköpings universitet