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High-Resolution XFE...
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Srinivas, VivekStockholms universitet,Institutionen för biokemi och biofysik
(author)
High-Resolution XFEL Structure of the Soluble Methane Monooxygenase Hydroxylase Complex with its Regulatory Component at Ambient Temperature in Two Oxidation States
- Article/chapterEnglish2020
Publisher, publication year, extent ...
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2020-07-20
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American Chemical Society (ACS),2020
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:su-185335
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-185335URI
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https://doi.org/10.1021/jacs.0c05613DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Soluble methane monooxygenase (sMMO)is a multicomponent metalloenzyme that catalyzes the conversion of methane to methanol at ambient temperature using a nonheme, oxygen-bridged dinuclear iron cluster in the active site. Structural changes in the hydroxylase component (sMMOH) containing the diiron cluster caused by complex formation with a regulatory component (MMOB) and by iron reduction are important for the regulation of O-2 activation and substrate hydroxylation. Structural studies of metalloenzymes using traditional synchrotron-based X-ray crystallography are often complicated by partial X-ray-induced photoreduction of the metal center, thereby obviating determination of the structure of the enzyme in pure oxidation states. Here, microcrystals of the sMMOH:MMOB complex from Methylosinus trichosporium OB3b were serially exposed to X-ray free electron laser (XFEL) pulses, where the <= 35 fs duration of exposure of an individual crystal yields diffraction data before photoreduction-induced structural changes can manifest. Merging diffraction patterns obtained from thousands of crystals generates radiation damage-free, 1.95 angstrom resolution crystal structures for the fully oxidized and fully reduced states of the sMMOH:MMOB complex for the first time. The results provide new insight into the manner by which the diiron cluster and the active site environment are reorganized by the regulatory protein component in order to enhance the steps of oxygen activation and methane oxidation. This study also emphasizes the value of XFEL and serial femtosecond crystallography (SFX) methods for investigating the structures of metalloenzymes with radiation sensitive metal active sites.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Banerjee, Rahul
(author)
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Lebrette, HugoStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)hlebr
(author)
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Jones, Jason C.
(author)
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Aurelius, OskarStockholms universitet,Institutionen för biokemi och biofysik
(author)
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Kim, In-Sik
(author)
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Pham, Cindy C.
(author)
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Gul, Sheraz
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Sutherlin, Kyle D.
(author)
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Bhowmick, Asmit
(author)
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John, JulianeStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)jujo3677
(author)
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Bozkurt, EsraStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)esbo4735
(author)
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Fransson, Thomas
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Aller, Pierre
(author)
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Butryn, Agata
(author)
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Bogacz, Isabel
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Simon, Philipp
(author)
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Keable, Stephen
(author)
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Britz, Alexander
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Tono, Kensuke
(author)
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Kim, Kyung Sook
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Park, Sang-Youn
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Lee, Sang Jae
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Park, Jaehyun
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Alonso-Mori, Roberto
(author)
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Fuller, Franklin D.
(author)
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Batyuk, Alexander
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Brewster, Aaron S.
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Bergmann, Uwe
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Sauter, Nicholas K.
(author)
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Orville, Allen M.
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Yachandra, Vittal K.
(author)
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Yano, Junko
(author)
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Lipscomb, John D.
(author)
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Kern, Jan
(author)
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Högbom, MartinStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)hogbom
(author)
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Stockholms universitetInstitutionen för biokemi och biofysik
(creator_code:org_t)
Related titles
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In:Journal of the American Chemical Society: American Chemical Society (ACS)142:33, s. 14249-142660002-78631520-5126
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- By the author/editor
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Srinivas, Vivek
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Banerjee, Rahul
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Lebrette, Hugo
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Jones, Jason C.
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Aurelius, Oskar
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Kim, In-Sik
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show more...
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Pham, Cindy C.
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Gul, Sheraz
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Sutherlin, Kyle ...
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Bhowmick, Asmit
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John, Juliane
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Bozkurt, Esra
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Fransson, Thomas
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Aller, Pierre
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Butryn, Agata
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Bogacz, Isabel
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Simon, Philipp
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Keable, Stephen
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Britz, Alexander
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Tono, Kensuke
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Kim, Kyung Sook
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Park, Sang-Youn
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Lee, Sang Jae
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Park, Jaehyun
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Alonso-Mori, Rob ...
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Fuller, Franklin ...
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Batyuk, Alexande ...
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Brewster, Aaron ...
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Bergmann, Uwe
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Sauter, Nicholas ...
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Orville, Allen M ...
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Yachandra, Vitta ...
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Yano, Junko
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Lipscomb, John D ...
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Kern, Jan
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Högbom, Martin
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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Stockholm University