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Differential protei...
Differential protein expression during growth on model and commercial mixtures of naphthenic acids in Pseudomonas fluorescens Pf-5
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McKew, Boyd A. (author)
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Johnson, Richard (author)
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Clothier, Lindsay (author)
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Skeels, Karl (author)
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Ross, Matthew S. (author)
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Metodiev, Metodi (author)
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Frenzel, Max (author)
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Gieg, Lisa M. (author)
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- Martin, Jonathan W. (author)
- Stockholms universitet,Institutionen för miljövetenskap
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Hough, Michael A. (author)
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Whitby, Corinne (author)
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(creator_code:org_t)
- 2021-07-19
- 2021
- English.
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In: MicrobiologyOpen. - : Wiley. - 2045-8827. ; 10:4
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https://doi.org/10.1...
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https://onlinelibrar...
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Abstract
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- Naphthenic acids (NAs) are carboxylic acids with the formula (CnH2n+ZO2) and are among the most toxic, persistent constituents of oil sands process-affected waters (OSPW), produced during oil sands extraction. Currently, the proteins and mechanisms involved in NA biodegradation are unknown. Using LC-MS/MS shotgun proteomics, we identified proteins overexpressed during the growth of Pseudomonas fluorescens Pf-5 on a model NA (4 '-n-butylphenyl)-4-butanoic acid (n-BPBA) and commercial NA mixture (Acros). By day 11, >95% of n-BPBA was degraded. With Acros, a 17% reduction in intensity occurred with 10-18 carbon compounds of the Z family -2 to -14 (major NA species in this mixture). A total of 554 proteins (n-BPBA) and 631 proteins (Acros) were overexpressed during growth on NAs, including several transporters (e.g., ABC transporters), suggesting a cellular protective response from NA toxicity. Several proteins associated with fatty acid, lipid, and amino acid metabolism were also overexpressed, including acyl-CoA dehydrogenase and acyl-CoA thioesterase II, which catalyze part of the fatty acid beta-oxidation pathway. Indeed, multiple enzymes involved in the fatty acid oxidation pathway were upregulated. Given the presumed structural similarity between alkyl-carboxylic acid side chains and fatty acids, we postulate that P. fluorescens Pf-5 was using existing fatty acid catabolic pathways (among others) during NA degradation.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- naphthenic acids
- oil sands process-affected water
- proteomics
- Pseudomonas fluorescens
- tailing ponds
- toxicity
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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McKew, Boyd A.
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Johnson, Richard
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Clothier, Lindsa ...
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Skeels, Karl
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Ross, Matthew S.
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Metodiev, Metodi
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show more...
-
Frenzel, Max
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Gieg, Lisa M.
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Martin, Jonathan ...
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Hough, Michael A ...
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Whitby, Corinne
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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MicrobiologyOpen
- By the university
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Stockholm University