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Multiplex protein a...
Multiplex protein analysis and ensemble machine learning methods of fine needle aspirates from prostate cancer patients reveal potential diagnostic signatures associated with tumour grade
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- Röbeck, Pontus (författare)
- Uppsala universitet,Urologkirurgi,Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden
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- Franzén, Bo (författare)
- Uppsala universitet,Karolinska Institutet,Urologkirurgi,Karolinska Inst, Dept Oncol & Pathol, Stockholm, Sweden
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- Cantera-Ahlman, Rafaele (författare)
- Uppsala universitet,Urologkirurgi
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- Dragomir, Anca (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Auer, Gert (författare)
- Karolinska Institutet,Karolinska Inst, Dept Oncol & Pathol, Stockholm, Sweden
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- Jorulf, Håkan (författare)
- Uppsala universitet,Radiologi
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- Jacobsson, Sven P. (författare)
- Stockholms universitet,Institutionen för analytisk kemi,Stockholm Univ, Dept Analyt Chem, Stockholm, Sweden
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- Viktorsson, Kristina (författare)
- Karolinska Institutet,Karolinska Inst, Dept Oncol & Pathol, Stockholm, Sweden
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- Lewensohn, Rolf (författare)
- Karolinska Inst, Dept Oncol & Pathol, Stockholm, Sweden.;Karolinska Univ Hosp, Thorac Oncol Ctr, Med Unit Head & Neck Lung & Skin Tumors, Theme Canc, Solna, Sweden
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- Häggman, Michael (författare)
- Uppsala universitet,Urologkirurgi
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- Ladjevardi, Sam (författare)
- Uppsala universitet,Urologkirurgi
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(creator_code:org_t)
- 2023-03-20
- 2023
- Engelska.
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Ingår i: Cytopathology. - : Wiley. - 0956-5507 .- 1365-2303. ; 34:4, s. 286-294
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Background: Improved molecular diagnosis is needed in prostate cancer (PC). Fine needle aspiration (FNA) is a minimally invasive biopsy technique, less traumatic compared to core needle biopsy, and could be useful for diagnosis of PC. Molecular biomarkers (BMs) in FNA-samples can be assessed for prediction, eg of immunotherapy efficacy before treatment as well as at treatment decision time points during disease progression.Methods: In the present pilot study, the expression levels of 151 BM proteins were analysed by proximity extension assay in FNA-samples from 16 patients, including benign prostate lesions (n = 3) and cancers (n = 13). An ensemble data analysis strategy was applied using several machine learning models.Results: Twelve potentially predictive BM proteins correlating with International Society of Urological Pathology grade groups were identified, among them vimentin, tissue factor pathway inhibitor 2, and integrin beta-5. The validity of the results was supported by network analysis that showed functional associations between most of the identified putative BMs. We also showed that multiple immune checkpoint targets can be assessed (eg PD-L1, CD137, and Galectin-9), which may support the selection of immunotherapy in advanced PC. Results are promising but need further validation in a larger cohort.Conclusions: Our pilot study represents a “proof of concept” and shows that multiplex profiling of potential diagnostic and predictive BM proteins is feasible on tumour material obtained by FNA sampling of prostate cancer. Moreover, our results demonstrate that an ensemble data analysis strategy may facilitate the identification of BM signatures in pilot studies when the patient cohort is limited.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- biomarkers
- fine needle aspiration biopsy
- immune signalling
- machine learning
- prostate cancer
- proximity extension assay
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Röbeck, Pontus
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Franzén, Bo
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Cantera-Ahlman, ...
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Dragomir, Anca
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Auer, Gert
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Jorulf, Håkan
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Jacobsson, Sven ...
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Viktorsson, Kris ...
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Lewensohn, Rolf
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Häggman, Michael
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Ladjevardi, Sam
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
- Artiklar i publikationen
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Cytopathology
- Av lärosätet
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Stockholms universitet
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Karolinska Institutet
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Uppsala universitet