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Rotary properties o...
Rotary properties of hybrid F1-ATPases consisting of subunits from different species
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Watanabe, Ryo R. (författare)
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- Tas Kiper, Busra (författare)
- Stockholms universitet,Matematiska institutionen
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Zarco-Zavala, Mariel (författare)
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Hara, Mayu (författare)
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Kobayashi, Ryohei (författare)
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Ueno, Hiroshi (författare)
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Garcia-Trejo, Jose J. (författare)
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- Li, Chun-Biu (författare)
- Stockholms universitet,Matematiska institutionen
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Noji, Hiroyuki (författare)
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(creator_code:org_t)
- 2023
- 2023
- Engelska.
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Ingår i: iScience. - 2589-0042. ; 26:5
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- F-1-ATPase (F-1) is an ATP-driven rotary motor protein ubiquitously found in many species as the catalytic portion of FoF1-ATP synthase. Despite the highly conserved amino acid sequence of the catalytic core subunits: alpha and beta, F-1 shows diversity in the maximum catalytic turnover rate V-max and the number of rotary steps per turn. To study the design principle of F-1, we prepared eight hybrid F(1)s composed of subunits from two of three genuine (F)1s: thermophilic Bacillus PS3 (TF1), bovine mitochondria (bMF(1)), and Paracoccus denitrificans (PdF1), differing in the V-max and the number of rotary steps. The V-max of the hybrids can be well fitted by a quadratic model highlighting the dominant roles of 0 and the couplings between alpha-beta. Although there exist no simple rules on which subunit dominantly determines the number of steps, our findings show that the stepping behavior is characterized by the combination of all subunits.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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