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Functional features...
Functional features of the C-terminal region of yeast ribosomal protein L5
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- Hossein, Moradi (författare)
- Södertörns högskola,Stockholms universitet,Wenner-Grens institut,Institutionen för livsvetenskaper,Stockholm University
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- Simoff, Ivailo (författare)
- Södertörns högskola,Stockholms universitet,Wenner-Grens institut,Institutionen för livsvetenskaper,Stockholm University
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- Bartish, Galyna (författare)
- Södertörns högskola,Stockholms universitet,Wenner-Grens institut,Institutionen för livsvetenskaper,Stockholm University
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- Nygård, Odd (författare)
- Södertörns högskola,Institutionen för livsvetenskaper
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(creator_code:org_t)
- 2008-08-27
- 2008
- Engelska.
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Ingår i: Molecular Genetics and Genomics. - : Springer Science and Business Media LLC. - 1617-4615 .- 1617-4623. ; 280:4, s. 337-350
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The aim of this study was to analyze the functional importance of the C-terminus of the essential yeast ribosomal protein L5 (YrpL5). Previous studies have indicated that the C-terminal region of YrpL5 forms an α-helix with a positively charged surface that is involved in protein–5S rRNA interaction. Formation of an YrpL5·5S rRNA complex is a prerequisite for nuclear import of YrpL5. Here we have tested the importance of the α-helix and the positively charged surface for YrpL5 function in Saccharomyces cerevisiae using site directed mutagenesis in combination with functional complementation. Alterations in the sequence forming the putative α-helix affected the functional capacity of YrpL5. However, the effect did not correlate with a decreased ability of the protein to bind to 5S rRNA as all rpL5 mutants tested were imported to the nucleus whether or not the α-helix or the positively charged surface were intact. The alterations introduced in the C-terminal sequence affected the growth rate of cells expressing mutant but functional forms of YrpL5. The reduced growth rate was correlated with a reduced ribosomal content per cell indicating that the alterations introduced in the C-terminus interfered with ribosome assembly.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Nyckelord
- Functional complementation
- Mutation analysis
- Ribosomal protein L5
- S. cerevisiae
- cellbiologi
- Cellbiology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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