Sökning: onr:"swepub:oai:DiVA.org:umu-106967" >
Vascular endothelia...
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Chien, Ming-Hsien
(författare)
Vascular endothelial growth factor-C (VEGF-C) promotes angiogenesis by induction of COX-2 in leukemic cells via the VEGF-R3/JNK/AP-1 pathway.
- Artikel/kapitelEngelska2009
Förlag, utgivningsår, omfång ...
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2009-10-13
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Oxford University Press (OUP),2009
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:umu-106967
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-106967URI
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https://doi.org/10.1093/carcin/bgp244DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Vascular endothelial growth factor (VEGF)-C is recognized as a tumor lymphangiogenic factor based on the effects of activated VEGF-R3 on lymphatic endothelial cells. Many tumor cells express VEGF-R3 but the function of this receptor in tumor cells is largely unknown. It has been reported that the VEGF-C/VEGF-R3 axis is activated in subsets of leukemia patients. Herein, we have shown that VEGF-C induces angiogenic activity in the tube formation assay invitro and Matrigel plug assay in vivo by upregulating an angiogenic factor, cyclooxygenase-2 (COX-2), through VEGF-R3 in the human acute myeloid leukemia (AML) cell line, THP-1. COX-2 induction by VEGF-C was also observed in other VEGF-R3(+) human AML cell lines (U937 and HL60). Moreover, immunohistochemical analysis of bone marrow specimens of 37 patients diagnosed with AML revealed that VEGF-C expression in specimens was associated with the expression of COX-2 (P < 0.001). The manner by which signaling pathways transduced by VEGF-C is responsible for COX-2 upregulation was further investigated. Blocking the p42/44 mitogen-activated protein kinase (MAPK) pathway with the MAPK kinase inhibitor, PD 98059, failed to inhibit VEGF-C-mediated COX-2 expression. However, VEGF-C-induced COX-2 upregulation was effectively abolished by overexpression of dominant-negative c-Jun N-terminal kinase (JNK) or treatment with the JNK inhibitor, SP 600125. VEGF-C induced JNK-dependent nuclear translocation of c-Jun. Furthermore, chromatin immunoprecipitation and reporter assays revealed that VEGF-C enhanced c-Jun binding to the cyclic adenosine 3',5'-monophosphate-response element of the COX-2 promoter and induced COX-2 expression. In sum, the data herein highlight the pathogenic role of VEGF-C in leukemia via regulation of angiogenesis through upregulation of COX-2.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Ku, Chia-Chi
(författare)
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Johansson, GunnarUmeå universitet,Onkologi,Umeå University(Swepub:umu)gurjon98
(författare)
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Chen, Min-Wei
(författare)
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Hsiao, Michael
(författare)
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Su, Jen-Liang
(författare)
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Inoue, Hiroyasu
(författare)
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Hua, Kuo-Tai
(författare)
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Wei, Lin-Hung
(författare)
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Kuo, Min-Liang
(författare)
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Umeå universitetOnkologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Carcinogenesis: Oxford University Press (OUP)30:12, s. 2005-130143-33341460-2180
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Till lärosätets databas
- Av författaren/redakt...
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Chien, Ming-Hsie ...
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Ku, Chia-Chi
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Johansson, Gunna ...
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Chen, Min-Wei
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Hsiao, Michael
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Su, Jen-Liang
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visa fler...
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Inoue, Hiroyasu
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Hua, Kuo-Tai
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Wei, Lin-Hung
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Kuo, Min-Liang
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Cellbiologi
- Artiklar i publikationen
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Carcinogenesis
- Av lärosätet
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Umeå universitet