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Dose-dependent inhi...
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Olsson, MattiasUmeå universitet,Histologi med cellbiologi
(författare)
Dose-dependent inhibitory effect of CD47 in macrophage uptake of IgG-opsonized murine erythrocytes.
- Artikel/kapitelEngelska2007
Förlag, utgivningsår, omfång ...
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Elsevier BV,2007
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:umu-12287
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-12287URI
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https://doi.org/10.1016/j.bbrc.2006.11.002DOI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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The cell surface glycoprotein CD47 on target cells can bind to the inhibitory receptor SIRPalpha on macrophages to inhibit phagocytosis of antibody sensitized blood cells. The aim of this study was to determine if CD47 dose-dependently can regulate macrophage uptake of IgG-opsonized RBCs. CD47(+/-) RBCs express about 50% of the CD47 level found on CD47(+/+) RBCs. When injected into CD47(+/+) mice, CD47(+/-) RBCs showed a significantly faster antibody-mediated clearance as compared with CD47(+/+) RBCs injected into the same recipient. In vitro phagocytosis experiments confirmed that CD47(+/-) RBCs were taken up significantly more than CD47(+/+) RBCs, but significantly less than CD47(-/-) RBCs. A reduction in RBC CD47 expression just below 50% of that in normal RBCs can significantly accelerate RBC clearance by macrophages in the presence of RBC autoantibodies. This may have relevance for transfusion of stored RBCs, where loss of CD47 is seen over time, and in clearance of these cells by antibody-dependent phagocytosis.
Ämnesord och genrebeteckningar
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Animals
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Antigens; CD47/genetics/*metabolism
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Cell Survival
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Erythrocytes/*immunology/*metabolism
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Female
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Gene Expression
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Immunoglobulin G/*immunology
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Macrophages/*immunology/*metabolism
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Male
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Mice
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Mice; Inbred BALB C
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Mice; Knockout
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Phagocytosis
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Nilsson, AnnaUmeå universitet,Histologi med cellbiologi(Swepub:umu)anastt99
(författare)
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Oldenborg, Per-ArneUmeå universitet,Histologi med cellbiologi(Swepub:umu)peol0001
(författare)
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Umeå universitetHistologi med cellbiologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Biochemical and Biophysical Research Communications - BBRC: Elsevier BV352:1, s. 193-1970006-291X1090-2104
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