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Effect of centrifug...
Effect of centrifugal force on the development of articular neocartilage with bovine primary chondrocytes
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- Prittinen, Juha, 1989- (författare)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Chondrogenic and osteogenic differentiation group
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- Ylärinne, Janne, 1988- (författare)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Chondrogenic and osteogenic differentiation group
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- Piltti, Juha (författare)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Nordlab Kokkola, Keski-Pohjanmaa Central Hospital Soite, Kokkola, Finland,Chondrogenic and osteogenic differentiation group
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- Karhula, Sakari S. (författare)
- Research Unit of Medical Imaging, Physics and Technology, University of Oulu, Oulu, Finland; Infotech Doctoral Program, University of Oulu, Oulu, Finland
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- Rieppo, Lassi (författare)
- Research Unit of Medical Imaging, Physics and Technology, University of Oulu, Oulu, Finland; Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Oulu, Finland
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- Ojanen, S. P. (författare)
- Research Unit of Medical Imaging, Physics and Technology, University of Oulu, Oulu, Finland; Department of Applied Physics, University of Eastern Finland, Kuopio, Finland
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- Korhonen, Rami K. (författare)
- Department of Applied Physics, University of Eastern Finland, Kuopio, Finland
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- Saarakkala, S. (författare)
- Research Unit of Medical Imaging, Physics and Technology, University of Oulu, Oulu, Finland; Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Oulu, Finland; Department of Diagnostic Radiology, Oulu University Hospital, Oulu, Finland
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- Lammi, Mikko, 1961- (författare)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an Jiaotong University, Xi'an, China,Chondrogenic and osteogenic differentiation group
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- Qu, Chengjuan, 1967- (författare)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Chondrogenic and osteogenic differentiation group
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(creator_code:org_t)
- 2018-10-23
- 2019
- Engelska.
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Ingår i: Cell and Tissue Research. - New York : Springer. - 0302-766X .- 1432-0878. ; 375:3, s. 629-639
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A lot has been invested into understanding how to assemble cartilage tissue in vitro and various designs have been developed to manufacture cartilage tissue with native-like biological properties. So far, no satisfactory design has been presented. Bovine primary chondrocytes are used to self-assemble scaffold-free constructs to investigate whether mechanical loading by centrifugal force would be useful in manufacturing cartilage tissue in vitro. Six million chondrocytes were laid on top of defatted bone disks placed inside an agarose well in 50-ml culture tubes. The constructs were centrifuged once or three times per day for 15 min at a centrifugal force of 771×g for up to 4 weeks. Control samples were cultured under the same conditions without exposure to centrifugation. The samples were analysed by (immuno)histochemistry, Fourier transform infrared imaging, micro-computed tomography, biochemical and gene expression analyses. Biomechanical testing was also performed. The centrifuged tissues had a more even surface covering a larger area of the bone disk. Fourier transform infrared imaging analysis indicated a higher concentration of collagen in the top and bottom edges in some of the centrifuged samples. Glycosaminoglycan contents increased along the culture, while collagen content remained at a rather constant level. Aggrecan and procollagen α1(II) gene expression levels had no significant differences, while procollagen α2(I) levels were increased significantly. Biomechanical analyses did not reveal remarkable changes. The centrifugation regimes lead to more uniform tissue constructs, whereas improved biological properties of the native tissue could not be obtained by centrifugation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Ortopedi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Orthopaedics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- Cartilage tissue engineering
- Centrifugal force
- Osteoarthritis
- Primary chondrocyte
- Tissue assembly
- Biochemistry
- biokemi
- biomechanics
- biomekanik
- cellforskning
- cell research
- Orthopaedics
- ortopedi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Prittinen, Juha, ...
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Ylärinne, Janne, ...
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Piltti, Juha
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Karhula, Sakari ...
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Rieppo, Lassi
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Ojanen, S. P.
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Korhonen, Rami K ...
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Saarakkala, S.
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Lammi, Mikko, 19 ...
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Qu, Chengjuan, 1 ...
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