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Analysis of Gram-ne...
Analysis of Gram-negative Bacteria Peptidoglycan by Ultra-performance Liquid Chromatography
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- Alvarez, Laura (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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Cordier, Baptiste (author)
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van Teeffelen, Sven (author)
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- Cava, Felipe (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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(creator_code:org_t)
- Bio-protocol, 2020
- 2020
- English.
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In: Bio-protocol. - : Bio-protocol. - 2331-8325. ; 10:19
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.2...
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Abstract
Subject headings
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- Bacteria are surrounded by a protective peptidoglycan cell wall. Provided that this structure and the enzymes involved are the preferred target for our most successful antibiotics, determining its structural and chemical complexity is of the highest interest. Traditionally, high-performance liquid chromatography (HPLC) analyses have been performed, but these methods are very time consuming in terms of sample preparation and chromatographic separation. Here we describe an optimized method for preparation of Gram-negative bacteria peptidoglycan and its subsequent analysis by ultra-performance liquid chromatography (UPLC). The use of UPLC in peptidoglycan analyses provides a dramatic reduction of the sample volume and hands-on time required and, furthermore, permits in-line mass spectrometry (MS) of the UPLC resolved muropeptides, thus facilitating their identification. This method improves our capability to perform high throughput analysis to better understand the cell- wall biology.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- UPLC
- Cell Wall
- Peptidoglycan
- Muropeptide
- Muramidase
- Crosslink
Publication and Content Type
- ref (subject category)
- art (subject category)
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