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EstG is a novel est...
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Daitch, Allison K.Department of Biological Chemistry, Johns Hopkins University School of Medicine, MD, Baltimore, United States
(author)
EstG is a novel esterase required for cell envelope integrity in Caulobacter
- Article/chapterEnglish2023
Publisher, publication year, extent ...
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Cell Press,2023
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Numbers
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LIBRIS-ID:oai:DiVA.org:umu-212079
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-212079URI
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https://doi.org/10.1016/j.cub.2022.11.037DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Proper regulation of the bacterial cell envelope is critical for cell survival. Identification and characterization of enzymes that maintain cell envelope homeostasis is crucial, as they can be targets for effective antibiotics. In this study, we have identified a novel enzyme, called EstG, whose activity protects cells from a variety of lethal assaults in the ⍺-proteobacterium Caulobacter crescentus. Despite homology to transpeptidase family cell wall enzymes and an ability to protect against cell-wall-targeting antibiotics, EstG does not demonstrate biochemical activity toward cell wall substrates. Instead, EstG is genetically connected to the periplasmic enzymes OpgH and BglX, responsible for synthesis and hydrolysis of osmoregulated periplasmic glucans (OPGs), respectively. The crystal structure of EstG revealed similarities to esterases and transesterases, and we demonstrated esterase activity of EstG in vitro. Using biochemical fractionation, we identified a cyclic hexamer of glucose as a likely substrate of EstG. This molecule is the first OPG described in Caulobacter and establishes a novel class of OPGs, the regulation and modification of which are important for stress survival and adaptation to fluctuating environments. Our data indicate that EstG, BglX, and OpgH comprise a previously unknown OPG pathway in Caulobacter. Ultimately, we propose that EstG is a novel enzyme that instead of acting on the cell wall, acts on cyclic OPGs to provide resistance to a variety of cellular stresses.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Orsburn, Benjamin C.Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, MD, Baltimore, United States
(author)
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Chen, ZanDepartment of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, MD, Baltimore, United States
(author)
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Alvarez, LauraUmeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Umeå Centre for Microbial Research (UCMR),Institutionen för molekylärbiologi (Medicinska fakulteten),Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet)(Swepub:umu)laal0007
(author)
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Eberhard, Colten D.Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, MD, Baltimore, United States
(author)
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Sundararajan, KousikDepartment of Biological Chemistry, Johns Hopkins University School of Medicine, MD, Baltimore, United States
(author)
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Zeinert, RileeDepartment of Biochemistry and Molecular Biology, University of Massachusetts-Amherst, MA, Amherst, United States
(author)
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Kreitler, Dale F.National Synchrotron Light Source II, Brookhaven National Laboratory, P.O. Box 5000, NY, Upton, United States
(author)
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Jakoncic, JeanNational Synchrotron Light Source II, Brookhaven National Laboratory, P.O. Box 5000, NY, Upton, United States
(author)
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Chien, PeterDepartment of Biochemistry and Molecular Biology, University of Massachusetts-Amherst, MA, Amherst, United States
(author)
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Cava, FelipeUmeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Umeå Centre for Microbial Research (UCMR),Institutionen för molekylärbiologi (Medicinska fakulteten)(Swepub:umu)feca0003
(author)
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Gabelli, Sandra B.Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe Street, MD, Baltimore, United States; Department of Oncology, Johns Hopkins University School of Medicine, 725 N Wolfe Street, MD, Baltimore, United States; Department of Medicine, Johns Hopkins University School of Medicine, MD, Baltimore, United States
(author)
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Goley, Erin D.Department of Biological Chemistry, Johns Hopkins University School of Medicine, MD, Baltimore, United States
(author)
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Department of Biological Chemistry, Johns Hopkins University School of Medicine, MD, Baltimore, United StatesDepartment of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, MD, Baltimore, United States
(creator_code:org_t)
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In:Current Biology: Cell Press33:2, s. 228-240.e70960-98221879-0445
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Zeinert, Rilee
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