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Functional Granuloc...
Functional Granulocyte–Macrophage Colony-Stimulating Factor (GM-CSF) Delivered by Canine Histiocytic Sarcoma Cells Persistently Infected with Engineered Attenuated Canine Distemper Virus
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- Marek, Katarzyna (författare)
- Department of Pathology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany; Center for Systems Neuroscience, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany
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- Armando, Federico (författare)
- Department of Pathology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany
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- Asawapattanakul, Thanaporn (författare)
- Department of Pathology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany; Center for Systems Neuroscience, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany
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- Nippold, Vanessa Maria (författare)
- Department of Pathology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany
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- Plattet, Philippe (författare)
- Division of Experimental Clinical Research, Vetsuisse University Bern, Bern, Switzerland
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- Gerold, Gisa, 1979- (författare)
- Umeå universitet,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Avdelningen för virologi,Department of Biochemistry, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany; Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Foundation, Hannover, Germany
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- Baumgärtner, Wolfgang (författare)
- Department of Pathology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany; Center for Systems Neuroscience, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany
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- Puff, Christina (författare)
- Department of Pathology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany
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(creator_code:org_t)
- MDPI, 2023
- 2023
- Engelska.
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Ingår i: Pathogens. - : MDPI. - 2076-0817. ; 12:7
- Relaterad länk:
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https://doi.org/10.3...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- The immune response plays a key role in the treatment of malignant tumors. One important molecule promoting humoral and cellular immunity is granulocyte–macrophage colony-stimulating factor (GM-CSF). Numerous successful trials have led to the approval of this immune-stimulating molecule for cancer therapy. However, besides immune stimulation, GM-CSF may also accelerate tumor cell proliferation, rendering this molecule a double-edged sword in cancer treatment. Therefore, detailed knowledge about the in vitro function of GM-CSF produced by infected tumor cells is urgently needed prior to investigations in an in vivo model. The aim of the present study was to functionally characterize a persistent infection of canine histiocytic sarcoma cells (DH82 cells) with the canine distemper virus strain Onderstepoort genetically engineered to express canine GM-CSF (CDV-Ondneon-GM-CSF). The investigations aimed (1) to prove the overall functionality of the virally induced production of GM-CSF and (2) to determine the effect of GM-CSF on the proliferation and motility of canine HS cells. Infected cells consistently produced high amounts of active, pH-stable GM-CSF, as demonstrated by increased proliferation of HeLa cells. By contrast, DH82 cells lacked increased proliferation and motility. The significantly increased secretion of GM-CSF by persistently CDV-Ondneon-GM-CSF-infected DH82 cells, the pH stability of this protein, and the lack of detrimental effects on DH82 cells renders this virus strain an interesting candidate for future studies aiming to enhance the oncolytic properties of CDV for the treatment of canine histiocytic sarcomas.
Ämnesord
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
Nyckelord
- canine distemper virus
- DH82 cells
- genetically engineered viruses
- GM-CSF
- histiocytic sarcoma
- viral oncolysis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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