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Identification and characterization of a TFIID-like multiprotein complex from Saccharomyces cerevisiae.
- Artikel/kapitelEngelska1995
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LIBRIS-ID:oai:DiVA.org:umu-42467
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-42467URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Although the mechanisms of transcriptional regulation by RNA polymerase II are apparently highly conserved from yeast to man, the identification of a yeast TATA-binding protein (TBP)-TBP-associated factor (TAFII) complex comparable to the metazoan TFIID component of the basal transcriptional machinery has remained elusive. Here, we report the isolation of a yeast TBP-TAFII complex which can mediate transcriptional activation by GAL4-VP16 in a highly purified yeast in vitro transcription system. We have cloned and sequenced the genes encoding four of the multiple yeast TAFII proteins comprising the TBP-TAFII multisubunit complex and find that they are similar at the amino acid level to both human and Drosophila TFIID subunits. Using epitope-tagging and immunoprecipitation experiments, we demonstrate that these genes encode bona fide TAF proteins and show that the yeast TBP-TAFII complex is minimally composed of TBP and seven distinct yTAFII proteins ranging in size from M(r) = 150,000 to M(r) = 25,000. In addition, by constructing null alleles of the cloned TAF-encoding genes, we show that normal function of the TAF-encoding genes is essential for yeast cell viability.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Bai, Y
(författare)
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Campbell, A M
(författare)
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Bjorklund, Stefan
(författare)
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Kim, Y J
(författare)
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Zhou, S
(författare)
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Kornberg, R D
(författare)
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Weil, P A
(författare)
Sammanhörande titlar
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Ingår i:Proceedings of the National Academy of Sciences of the United States of America92:18, s. 8224-80027-84241091-6490
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