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Motor-free mitochon...
Motor-free mitochondrial presequence translocase drives membrane integration of preproteins
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van der Laan, Martin (author)
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Meinecke, Michael (author)
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Dudek, Jan (author)
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Hutu, Dana P. (author)
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- Lind, Maria (author)
- Uppsala universitet,Jämförande fysiologi
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Perschil, Inge (author)
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Guiard, Bernard (author)
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Wagner, Richard (author)
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Pfanner, Nikolaus (author)
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Rehling, Peter (author)
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(creator_code:org_t)
- 2007-09-09
- 2007
- English.
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In: Nature Cell Biology. - : Springer Science and Business Media LLC. - 1465-7392 .- 1476-4679. ; 9:10, s. 1152-1159
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- The mitochondrial inner membrane is the central energy-converting membrane of eukaryotic cells. the electrochemical proton gradient generated by the respiratory chain drives the ATP synthase. to maintain this proton-motive force, the inner membrane forms a tight barrier and strictly controls the translocation of ions(1). However, the major preprotein transport machinery of the inner membrane, termed the presequence translocase, translocates polypeptide chains into or across the membrane(2-9). Different views exist of the molecular mechanism of the translocase, in particular of the coupling with the import motor of the matrix(8,10,11). Wehave reconstituted preprotein transport into the mitochondrial inner membrane by incorporating the purified presequence translocase into cardiolipin-containing liposomes. We show that the motor-free form of the presequence translocase integrates preproteins into the membrane. the reconstituted presequence translocase responds to targeting peptides and mediates voltage-driven preprotein translocation, lateral release and insertion into the lipid phase. thus, the minimal system for preprotein integration into the mitochondrial inner membrane is the presequence translocase, a cardiolipin-rich membrane and a membrane potential.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Biology
- Biologi
- Chemistry
- Kemi
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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van der Laan, Ma ...
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Meinecke, Michae ...
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Dudek, Jan
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Hutu, Dana P.
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Lind, Maria
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Perschil, Inge
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show more...
-
Guiard, Bernard
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Wagner, Richard
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Pfanner, Nikolau ...
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Rehling, Peter
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
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Nature Cell Biol ...
- By the university
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Uppsala University