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Polypeptide Folding...
Polypeptide Folding-Mediated Tuning of the Optical and Structural Properties of Gold Nanoparticle Assemblies
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- Aili, Daniel (författare)
- University of London Imperial College of Science Technology and Medicine
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- Gryko, Piotr (författare)
- University of London Imperial College of Science Technology and Medicine
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- Sepulveda, Borja (författare)
- Research Centre Nanosci and Nanotechnol CIN2 CSIC
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- Dick, John A. G. (författare)
- University of London Imperial College of Science Technology and Medicine
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- Kirby, Nigel (författare)
- Australian Synchrotron
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- Heenan, Richard (författare)
- Rutherford Appleton Lab
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- Baltzer, Lars (författare)
- Uppsala universitet,Institutionen för biokemi och organisk kemi,Uppsala University
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- Liedberg, Bo (författare)
- Linköpings universitet,Sensorvetenskap och Molekylfysik,Tekniska högskolan
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- Ryan, Mary P. (författare)
- University of London Imperial College of Science Technology and Medicine
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- Stevens, Molly M. (författare)
- University of London Imperial College of Science Technology and Medicine
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(creator_code:org_t)
- 2011-11-08
- 2011
- Engelska.
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Ingår i: Nano letters (Print). - : American Chemical Society (ACS). - 1530-6984 .- 1530-6992. ; 11:12, s. 5564-5573
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Responsive hybrid nanomaterials with well-defined properties are of significant interest for the development of biosensors with additional applications in tissue engineering and drug delivery. Here, we present a detailed characterization using UV-vis spectroscopy and small angle X-ray scattering of a hybrid material comprised of polypeptide-decorated gold nanoparticles with highly controllable assembly properties. The assembly is triggered by a folding-dependent bridging of the particles mediated by the heteroassociation of immobilized helix-loop-helix polypeptides and a complementary nonlinear polypeptide present in solution. The polypeptides are de novo designed to associate and fold into a heterotrimeric complex comprised of two disulfide-linked four-helix bundles. The particles form structured assemblies with a highly defined interparticle gap (4.8 +/- 0.4 nm) that correlates to the size of the folded polypeptides. Transitions in particle aggregation dynamics, mass-fractal dimensions and ordering, as a function of particle size and the concentration of the bridging polypeptide, are observed; these have significant effects on the optical properties of the assemblies. The assembly and ordering of the particles are highly complex processes that are affected by a large number of variables including the number of polypeptides bridging the particles and the particle mobility within the aggregates. A fundamental understanding of these processes is of paramount interest for the development of novel hybrid nanomaterials with tunable structural and optical properties and for the optimization of nanoparticle-based colorimetric biodetection strategies.
Nyckelord
- Gold nanoparticles
- polypeptides
- folding
- helix-loop-helix
- small angle X-ray scattering (SAXS)
- MEDICINE
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
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Aili, Daniel
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Gryko, Piotr
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Sepulveda, Borja
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Dick, John A. G.
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Kirby, Nigel
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Heenan, Richard
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visa fler...
-
Baltzer, Lars
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Liedberg, Bo
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Ryan, Mary P.
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Stevens, Molly M ...
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visa färre...
- Artiklar i publikationen
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Nano letters (Pr ...
- Av lärosätet
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Uppsala universitet
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Linköpings universitet