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A proposed time-res...
A proposed time-resolved X-ray scattering approach to track local and global conformational changes in membrane transport proteins
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- Andersson, Magnus (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Vincent, Jonathan (author)
- Uppsala universitet,Institutionen för fotokemi och molekylärvetenskap,Uppsala University,Uppsala Univ, Dept Photochem & Mol Sci
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- van der Spoel, David (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi,Uppsala University
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- Davidsson, Jan (author)
- Uppsala universitet,Institutionen för fotokemi och molekylärvetenskap,Uppsala University
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- Neutze, Richard, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
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(creator_code:org_t)
- Elsevier BV, 2008
- 2008
- English.
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In: Structure. - : Elsevier BV. - 0969-2126 .- 1878-4186. ; 16:1, s. 21-28
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Abstract
Subject headings
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- Time-resolved X-ray scattering has emerged as a powerful technique for studying the rapid structural dynamics of small molecules in solution. Membrane-protein-catalyzed transport processes frequently couple large-scale conformational changes of the transporter with local structural changes perturbing the uptake and release of the transported substrate. Using light-driven halide ion transport catalyzed by halorhodopsin as a model system, we combine molecular dynamics simulations with X-ray scattering calculations to demonstrate how small-molecule time-resolved X-ray scattering can be extended to the study of membrane transport processes. In particular, by introducing strongly scattering atoms to label specific positions within the protein and substrate, the technique of time-resolved wide-angle X-ray scattering can reveal both local and global conformational changes. This approach simultaneously enables the direct visualization of global rearrangements and substrate movement, crucial concepts that underpin the alternating access paradigm for membrane transport proteins.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Data- och informationsvetenskap (hsv//swe)
- NATURAL SCIENCES -- Computer and Information Sciences (hsv//eng)
Keyword
- PROTEIN
- Biology
- Biologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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