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Identification of T...
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Smith, Casey Jo Anne
(författare)
Identification of TPIT and other novel autoantigens in lymphocytic hypophysitis : immunoscreening of a pituitary cDNA library and development of immunoprecipitation assays
- Artikel/kapitelEngelska2012
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LIBRIS-ID:oai:DiVA.org:uu-173821
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-173821URI
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https://doi.org/10.1530/EJE-11-1015DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:124335079URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Background: Lymphocytic hypophysitis is an organ-specific autoimmune disease of the pituitary gland. A specific and sensitive serological test currently does not exist to aid in the diagnosis. Objective: To identify target autoantigens in lymphocytic hypophysitis and develop a diagnostic assay for these proteins. Design/methods: A pituitary cDNA expression library was immunoscreened using sera from four patients with lymphocytic hypophysitis. Relevant cDNA clones from screening, along with previously identified autoantigens pituitary gland-specific factor 1a and 2 (PGSF1a and PGSF2) and neuron-specific enolase (NSE) were tested in an in vitro transcription and translation immunoprecipitation assay. The corticotroph-specific transcription factor, TPIT, was investigated separately as a candidate autoantigen. Results: Significantly positive autoantibody reactivity against TPIT was found in 9/86 hypophysitis patients vs 1/90 controls (P = 0.018). The reactivity against TPIT was not specific for lymphocytic hypophysitis with autoantibodies detectable in the sera from patients with other autoimmune endocrine diseases. Autoantibodies were also detected against chromodomain-helicase-DNA binding protein 8, presynaptic cytomatrix protein (piccolo), Ca2+-dependent secretion activator, PGSF2 and NSE in serum samples from patients with lymphocytic hypophysitis, but at a frequency that did not differ from healthy controls. Importantly, 8/86 patients with lymphocytic hypophysitis had autoantibodies against any two autoantigens in comparison with 0/90 controls (P = 0.0093). Conclusions: TPIT, a corticotroph-specific transcription factor, was identified as a target autoantigen in 10.5% of patients with lymphocytic hypophysitis. Further autoantigens related to vesicle processing were also identified as potential autoantigens with different immunoreactivity patterns in patients and controls.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Bensing, SophieKarolinska Institutet,Uppsala universitet,Institutionen för medicinska vetenskaper(Swepub:uu)sopbe271
(författare)
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Burns, Christine
(författare)
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Robinson, Phillip J.
(författare)
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Kasperlik-Zaluska, Anna A.
(författare)
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Scott, Rodney J.
(författare)
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Kämpe, OlleUppsala universitet,Institutionen för medicinska vetenskaper(Swepub:uu)ollekamp
(författare)
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Crock, Patricia A.
(författare)
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Uppsala universitetInstitutionen för medicinska vetenskaper
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:European Journal of Endocrinology166:3, s. 391-3980804-46431479-683X
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