Artificially designed promoters: understanding the role of spatial features and canonical binding sites in transcription

Artificially designed promoters : understanding the role of spatial features and canonical binding sites in transcription

Baumann, Martina (author): Univ Nat Resources & Appl Life Sci, Dept Biotechnol, Vienna, Austria

Höppner, Marc P. (author): Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Science for Life Laboratory, SciLifeLab

Meier, Michael (author): Univ Nat Resources & Appl Life Sci, Dept Biotechnol, Vienna, Austria

Pontiller, Jens (author): Univ Nat Resources & Appl Life Sci, Dept Biotechnol, Vienna, Austria

Ernst, Wolfgang (author): Univ Nat Resources & Appl Life Sci, Dept Biotechnol, Vienna, Austria

Grabherr, Reingard (author): Univ Nat Resources & Appl Life Sci, Dept Biotechnol, Vienna, Austria

Grabherr, Manfred G. (author): Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Science for Life Laboratory, SciLifeLab,Broad Inst, Cambridge, MA USA

(creator_code:org_t)

2014-10-27
2012
English.
In: Bioengineered Bugs. - : Informa UK Limited. - 1949-1018 .- 1949-1026. ; 3:2, s. 120-123

Related links:: https://www.tandfonl...; show more...; https://urn.kb.se/re...; https://doi.org/10.4...; show less...

Abstract Subject headings

The promoter is a key element in gene transcription and regulation. We previously reported that artificial sequences rich in the dinucleotide CpG are sufficient to drive expression in vitro in mammalian cell lines, without requiring canonical binding sites for transcription factor proteins. Here, we report that introducing a promoter organization that alternates in CpGs and regions rich in A and T further increases expression strength, as well as how insertion of specific binding sites makes such sequences respond to induced levels of the transcription factor NFκB. Our findings further contribute to the mechanistic understanding of promoters, as well as how these sequences might be shaped by evolutionary pressure in living organisms.

By the author/editor: Baumann, Martina; Höppner, Marc P.; Meier, Michael; Pontiller, Jens; Ernst, Wolfgang; Grabherr, Reinga ...; show more...; Mauceli, Evan; Grabherr, Manfre ...; show less...

About the subject

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