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Circulating immune ...
Circulating immune complexes (IC) and IC-induced levels of GM-CSF are increased in sudanese patients with acute visceral Leishmania donovani infection undergoing sodium stibogluconate treatment : implications for disease pathogenesis
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- Elshafie, Amir, 1970- (författare)
- Uppsala universitet,Klinisk immunologi,Enheten för klinisk immunologi,Autoimmunitet
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- Åhlin, Erik (författare)
- Uppsala universitet,Klinisk immunologi,Enheten för klinisk immunologi,Autoimmunitet
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- Mathsson, Linda (författare)
- Uppsala universitet,Klinisk immunologi,Enheten för klinisk immunologi,Autoimmunitet
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EIGhazali, Gehad (författare)
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- Rönnelid, Johan (författare)
- Uppsala universitet,Klinisk immunologi,Enheten för klinisk immunologi,Autoimmunitet
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(creator_code:org_t)
- 2007
- 2007
- Engelska.
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Ingår i: Journal of Immunology. - 0022-1767 .- 1550-6606. ; 178:8, s. 5383-5389
- Relaterad länk:
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http://www.jimmunol....
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https://urn.kb.se/re...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Infection with Leishmania donovani is associated with IL-10 as well as with GM-CSF. Immune complexes (IC) exert important functions by stimulation of monocytes/macrophage-mediated production of pro- and anti-inflammatory cytokines in rheumatic diseases. In this investigation, we have explored IC-induced cytokine production during Leishmania infection. Sera from 43 patients with visceral leishmaniasis (VL), 17 patients with post-kala-azar dermal leishmaniasis, and 20 healthy Sudanese controls were precipitated with polyethylene glycol (PEG). The PEG precipitates were added to serum-free PBMC for 20 h,whereupon supernatant levels of IL-1beta, IL-6, IL-10, IL-1 receptor antagonist protein, TNF-alpha, TNF receptor p75, and GM-CSF were investigated using ELISA. Circulating levels of C1q-binding IC were also measured in the serum samples. PEG precipitates from Leishmania-infected patients induced significantly higher levels of GM-CSF (p = 0.0037) and IL-10 (p < 0.0001), as well as of IL-6 (p < 0.0001) and IL-1 receptor antagonist (p = 0.0238) as compared with PEG precipitates from controls. Patients with acute VL as well as VL patients receiving sodium stibogluconate treatment displayed significantly increased levels of PEG precipitate-induced GM-CSF. The induction of GM-CSF by circulating IC was especially prominent in acute VL patients receiving sodium stibogluconate treatment; ANOVA revealed significant interaction between disease activity and treatment for PEG precipitate-induced levels of GM-CSF (disease activity, p = 0.0006; treatment, p = 0.0005; interaction, p = 0.0046). Parallel associations were determined for C1q-binding immune complexes, but not for any cytokine other than GM-CSF. The importance of IC-induced GM-CSF in leishmaniasis warrants further study.
Nyckelord
- Klinisk immunologi
- Clinical Immunology
- MEDICINE
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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