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Knock-down of ZBED6...
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Wang, Xuan,1984-Uppsala universitet,Institutionen för medicinsk cellbiologi,Science for Life Laboratory, SciLifeLab
(författare)
Knock-down of ZBED6 in insulin-producing cells promotes N-cadherin junctions between beta-cells and neural crest stem cells in vitro
- Artikel/kapitelEngelska2016
Förlag, utgivningsår, omfång ...
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2016-01-11
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Springer Science and Business Media LLC,2016
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-223614
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-223614URI
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https://doi.org/10.1038/srep19006DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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The role of the novel transcription factor ZBED6 for the adhesion/clustering of insulin-producing mouse MIN6 and βTC6 cells was investigated. Zbed6-silencing in the insulin producing cells resulted in increased three-dimensional cell-cell clustering and decreased adhesion to mouse laminin and human laminin 511. This was paralleled by a weaker focal adhesion kinase phosphorylation at laminin binding sites. Zbed6-silenced cells expressed less E-cadherin and more N-cadherin at cell-to-cell junctions. A strong ZBED6-binding site close to the N-cadherin gene transcription start site was observed. Three-dimensional clustering in Zbed6-silenced cells was prevented by an N-cadherin neutralizing antibody and by N-cadherin knockdown. Co-culture of neural crest stem cells (NCSCs) with Zbed6-silenced cells, but not with control cells, stimulated the outgrowth of NCSC processes. The cell-to-cell junctions between NCSCs and βTC6 cells stained more intensely for N-cadherin when Zbed6-silenced cells were co-cultured with NCSCs. We conclude that ZBED6 decreases the ratio between N- and E-cadherin. A lower N- to E-cadherin ratio may hamper the formation of three-dimensional beta-cell clusters and cell-to-cell junctions with NCSC, and instead promote efficient attachment to a laminin support and monolayer growth. Thus, by controlling beta-cell adhesion and cell-to-cell junctions, ZBED6 might play an important role in beta-cell differentiation, proliferation and survival.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Xie, BeichenUppsala universitet,Institutionen för medicinsk cellbiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)beixi713
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Qi, YuUppsala universitet,Institutionen för medicinsk cellbiologi,Science for Life Laboratory, SciLifeLab
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Wallerman, OlaUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)olwal516
(författare)
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Vasylovska, SvitlanaUppsala universitet,Regenerativ neurobiologi(Swepub:uu)sviva947
(författare)
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Andersson, LeifUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)leand227
(författare)
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Kozlova, ElenaUppsala universitet,Regenerativ neurobiologi(Swepub:uu)elenkozl
(författare)
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Welsh, NilsUppsala universitet,Institutionen för medicinsk cellbiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)nilswels
(författare)
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Uppsala universitetInstitutionen för medicinsk cellbiologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Scientific Reports: Springer Science and Business Media LLC62045-2322
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