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A Cactus-Derived To...
A Cactus-Derived Toxin-Like Cystine Knot Peptide with Selective Antimicrobial Activity
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- Aboye, Teshome L. (author)
- Uppsala universitet,Avdelningen för farmakognosi,Ulf Goransson
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- Strömstedt, Adam A. (author)
- Uppsala universitet,Avdelningen för farmakognosi,Ulf Goransson
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- Gunasekera, Sunithi (author)
- Uppsala universitet,Avdelningen för farmakognosi,Ulf Goransson
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- Bruhn, Jan G. (author)
- Uppsala universitet,Avdelningen för farmakognosi
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- El-Seedi, Hesham (author)
- Uppsala universitet,Avdelningen för farmakognosi,Ulf Goransson
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Rosengren, K. Johan (author)
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- Göransson, Ulf (author)
- Uppsala universitet,Avdelningen för farmakognosi,Ulf Goransson
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(creator_code:org_t)
- 2015-03-27
- 2015
- English.
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In: ChemBioChem. - : Wiley. - 1439-4227 .- 1439-7633. ; 16:7, s. 1068-1077
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Naturally occurring cystine knot peptides show a wide range of biological activity, and as they have inherent stability they represent potential scaffolds for peptide-based drug design and biomolecular engineering. Here we report the discovery, sequencing, chemical synthesis, three-dimensional solution structure determination and bioactivity of the first cystine knot peptide from Cactaceae (cactus) family: Ep-AMP1 from Echinopsis pachanoi. The structure of Ep-AMP1 (35 amino acids) conforms to that of the inhibitor cystine knot (or knottin) family but represents a novel diverse sequence; its activity was more than 500 times higher against bacterial than against eukaryotic cells. Rapid bactericidal action and liposome leakage implicate membrane permeabilisation as the mechanism of action. Sequence homology places Ec-AMP1 in the plant C6-type of antimicrobial peptides, but the three dimensional structure is highly similar to that of a spider neurotoxin.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
Keyword
- antibiotics
- C6-type AMP
- Cactaceae
- cystine knot
- drug discovery
- plant antimicrobial peptide
Publication and Content Type
- ref (subject category)
- art (subject category)
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