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Towards phasing usi...
Towards phasing using high X-ray intensity
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Galli, Lorenzo (author)
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Son, Sang-Kil (author)
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Barends, Thomas R. M. (author)
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White, Thomas A. (author)
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Barty, Anton (author)
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Botha, Sabine (author)
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Boutet, Sébastien (author)
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- Caleman, Carl (author)
- Uppsala universitet,Molekyl- och kondenserade materiens fysik
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Doak, R. Bruce (author)
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Nanao, Max H. (author)
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Nass, Karol (author)
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Shoeman, Robert L. (author)
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- Timneanu, Nicusor (author)
- Uppsala universitet,Molekylär biofysik,Molekyl- och kondenserade materiens fysik
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Santra, Robin (author)
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Schlichting, Ilme (author)
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Chapman, Henry N. (author)
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(creator_code:org_t)
- 2015
- 2015
- English.
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In: IUCrJ. - 2052-2525. ; 2, s. 627-634
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Abstract
Subject headings
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- X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential ‘bleaching’ of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed.
Subject headings
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
Keyword
- serial femtosecond crystallography
- high-intensity phasing
- radiation damage
- electronic damage
- X-ray free-electron lasers
- high XFEL doses
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Galli, Lorenzo
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Son, Sang-Kil
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Barends, Thomas ...
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White, Thomas A.
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Barty, Anton
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Botha, Sabine
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show more...
-
Boutet, Sébastie ...
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Caleman, Carl
-
Doak, R. Bruce
-
Nanao, Max H.
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Nass, Karol
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Shoeman, Robert ...
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Timneanu, Nicuso ...
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Santra, Robin
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Schlichting, Ilm ...
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Chapman, Henry N ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Structural Biolo ...
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IUCrJ
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Uppsala University