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Choice of molecular...
Choice of molecular barcode will affect species prevalence but not bacterial community composition
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- Lebret, Karen (författare)
- Uppsala universitet,Limnologi,Marine Biol Assoc UK, Citadel Hill, Plymouth, Devon, England
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- Schroeder, Joanna (författare)
- Marine Biol Assoc UK, Citadel Hill, Plymouth, Devon, England
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- Balestreri, Cecilia (författare)
- Marine Biol Assoc UK, Citadel Hill, Plymouth, Devon, England
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- Highfield, Andrea (författare)
- Marine Biol Assoc UK, Citadel Hill, Plymouth, Devon, England
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- Cummings, Denise (författare)
- Plymouth Marine Lab, Prospect Pl, Plymouth, Devon, England
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- Smyth, Tim (författare)
- Plymouth Marine Lab, Prospect Pl, Plymouth, Devon, England
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- Schroeder, Declan (författare)
- Marine Biol Assoc UK, Citadel Hill, Plymouth, Devon, England
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(creator_code:org_t)
- Elsevier BV, 2016
- 2016
- Engelska.
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Ingår i: Marine Genomics. - : Elsevier BV. - 1874-7787 .- 1876-7478. ; 29, s. 39-43
- Relaterad länk:
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https://uu.diva-port... (primary) (Raw object)
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Abstract The rapid advancement of next generation sequencing protocols in recent years has led to the diversification in the methods used to study microbial communities; however, how comparable the data generated from these different methods are, remains unclear. In this study we compared the taxonomic composition and seasonal dynamics of the bacterial community determined by two distinct 16s amplicon sequencing protocols: sequencing of the V6 region of the 16s rRNA gene using 454 pyrosequencing vs the V4 region of the 16s rRNA gene using the Illumina Hiseq 2500 platform. Significant differences between relative abundances at all taxonomic levels were observed; however, their seasonal dynamics between phyla were largely consistent between methods. This study highlights that care must be taken when comparing datasets generated from different methods.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
- NATURVETENSKAP -- Geovetenskap och miljövetenskap -- Oceanografi, hydrologi och vattenresurser (hsv//swe)
- NATURAL SCIENCES -- Earth and Related Environmental Sciences -- Oceanography, Hydrology and Water Resources (hsv//eng)
Nyckelord
- Marine bacterial community
- 16S
- Pyrosequencing
- Illumina
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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